An inflammatory model of dry eye syndrome using scopolamine

International audience

A pro-diabetic diet triggers early functional and structural changes in the rat retina

International audience; Purpose : Diabetic retinopathy (DR) is the leading cause of blindness in industrial countries before the age of 50 years. The early consequences of diabetes on the retina are nevertheless poorly known. We therefore aimed at characterizing the early effects of a high fructose and high fat diet on the function and structure of the rat retina. Methods : Male Brown Norway rats (6 weeks of age) were fed for 8 days (n=16), 4 weeks (n=16) and 12 weeks (n=8) with 60% fructose+10% lipid rich diet (HFHF), or a standard chow (n=8). At each time point, intraperitoneal insulin tolerance test (ITT-0.5 U/ml) and intraperitoneal glucose tolerance test (GTT-2g/kg body weight) were ca…

Time Course of Inflammation and Apoptosis in a Scopolamine–Induced Dry Eye Rat Model

International audience; Purpose: : Dry eye syndrome is due to tear secretion deficiency or excessive tear evaporation leading to tear film instability. The aim of this study was to evaluate the time course of inflammation and apoptosis in a scopolamine–induced dry eye rat model. Methods: : Osmotic pumps continuously delivering scopolamine (12.5mg/d) were implanted subcutaneously in female Lewis rats for 1, 2, 3, 7, 10, 17 and 28 days. During the treatment, the corneal status was assessed by fluorescein impregnation and tears were collected for prostaglandin E2 (PGE2) immunoassay. At the end of the treatment period, the animals were sacrificed and the ocular tissues and the lacrimal glands w…

The ApoB100,LDLR-/- mouse model as an experimental model to study aging of the retina: focus on autophagy, inflammation and oxidative stress

International audience

High fat-high fructose diet triggered metabolic syndrome and retinal neovascular complications in the rat

This is an abstract that was submitted for the 2016 ARVO Annual Meeting, held in Seattle, Wash., May 1-5, 2016.; Purpose : On the contrary to type II diabetes, the consequences of metabolic syndrome (MetS) in the retina are poorly documented. MetS includes insulin resistance, glucose intolerance, dyslipidemia and abdominal adiposity. Vascular changes are strongly associated to metabolic diseases. Thus, we tested the hypothesis that feeding rats with high fructose (60%) and high fat (10%) would contribute to the development of MetS and neovascular retinal complications.Methods : Male Brown Norway rats (6 weeks of age) were fed with a regular chow diet or a 60% rich fructose+10% lipid-rich di…

Mice display cholesterol deposits in the retina and reduced electroretinographic response

International audience

Effet chronique d’un édulcorant non nutritif – le sucralose - sur la détection hypothalamique du glucose chez le rat

Adhesion of salivary mucins (MUC5B) to human buccal cells as affected by a taste solution

National audience

Role of Oxidative Stress in Aging of the Retina in the ApoB100,LDLR-/- Mouse, a Murine Model of Aging of the Human Retina

Dietary superoxide dismutase protects against light-induced retinal oxidative stress in young senescence accelerated mice (SAM)

International audience

Evaluation of an experimental model of dry eye in the rat

International audience

Characterization of cholesterol metabolism in an experimental model of glaucoma in rats

International audience; Glaucoma is a neurodegenerative pathology characterized by retinal ganglion cell death. Cholesterol homeostasis disruption has been implicated in several neurodegenerative diseases such as Alzheimer and Huntington. However, its specific role in the retina and association with glaucoma remain unclear. The aim of this study was to characterize the modifications of cholesterol metabolism occurring during an experimental model of glaucoma in rats. Eight-weeks old male rats were subjected to laser photocoagulation of episcleral veins, limbus and trabecular meshwork to elevate intraocular pressure. At a short (3 days) and a long (2 months) time period post-laser, the retin…

Steady-state levels of retinal 24S-hydroxycholesterol are maintained by glial cells intervention after elevation of intraocular pressure in the rat

Purpose: Our previous studies suggested that CYP46A1 and 24S-hydroxycholesterol. (24SOH) may be associated with glaucoma. Loss of CYP46A1-expressing retinal. ganglion cells is involved in the activation of glia, and therefore possibly in the. disbalance of cholesterol. In this context, the purpose of our present work was to. emphasize the glial and longitudinal CYP46A1 expression after an interventional. glaucoma-related stress triggered by elevated intraocular pressure (IOP). Methods: Sprague–Dawley rats were submitted to laser photocoagulation of the. trabecular meshwork, limbus and episcleral veins in one eye to induce elevated IOP. Rats were euthanized at days 3, 14, 30 and 60 (n = 10 p…