0000000000476504

AUTHOR

M. Sánchez-hervás

showing 2 related works from this author

Mycobiota and mycotoxin producing fungi from cocoa beans.

2008

The present study reports on the natural mycobiota occurring in cocoa beans, paying special attention to the incidence of fungal species that are potential producers of mycotoxins. The results show that predominant fungi were different species of the genus Aspergillus belonging to section Flavi and Nigri. Of the 214 strains of Aspergillus section Flavi collected from cocoa beans, 120 were identified as A. flavus and 94 as A. tamarii. Of Aspergillus section Nigri 138 strains were isolated, with 132 belonging to A. niger aggregate and 6 to A. carbonarius species. Potential ability to produce aflatoxins (AFs) B1, B2, G1 and G2, cyclopiazonic acid (CPA) and ochratoxin A (OTA) was studied by iso…

Ochratoxin AAflatoxinMycobiotaAspergillus flavusFood ContaminationMicrobiologyRisk Assessmentchemistry.chemical_compoundBotanyHumansheterocyclic compoundsFood scienceMycotoxinChromatography High Pressure LiquidAspergillusCacaobiologyAspergillus nigerfood and beveragesGeneral MedicineMycotoxinsbiology.organism_classificationOchratoxinsAspergilluschemistryConsumer Product SafetyAspergillus nigerCyclopiazonic acidFood ScienceAspergillus flavusInternational journal of food microbiology
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Molecular characterization of the black Aspergillus isolates responsible for ochratoxin A contamination in grapes and wine in relation to taxonomy of…

2009

This work examines ochratoxigenic mycobiota in grapes by ap-PCR analysis sequence analysis of the ITS and IGS regions and ability to produce OTA. A comparison was also made with many reference strains of Aspergillus section Nigri. Based on ap-PCR profiles, derived from two microsatellite primers, three main groups were obtained by UPGMA cluster analysis corresponding to A. carbonarius, A. niger and A. tubingensis. The cophenetic correlation values corresponding to ap-PCR UPGMA analysis revealed a higher genetic variability in A. niger and A. tubingensis than in A. carbonarius. In addition, no genotypical differences could be established between OTA producers and nonproducers in all species …

Ochratoxin AMycobiotaPhylogenetic treeSequence analysisUPGMAGenetic VariationGeneral MedicineBiologyMicrobiologyOchratoxinsPolymerase Chain Reactionchemistry.chemical_compoundchemistryBotanyGenetic variationDNA Ribosomal SpacerFood MicrobiologyCluster AnalysisVitisGenetic variabilityAspergillus nigerDNA FungalOchratoxinPhylogenyFood ScienceInternational journal of food microbiology
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