0000000000481487
AUTHOR
B. Rinkevich
The multixenobiotic resistance mechanism in the marine sponge Suberites domuncula: its potential applicability for the evaluation of environmental pollution by toxic compounds
Experiments were carried out with the marine sponge Suberites domuncala to determine whether sponges may express - like mammalian tumor cells a multidrug-like transporter system. The results demonstrate that sponge cells possess such a protective system termed multixenobiotic resistance (MXR) pump or P-glycoprotein-like pump, The protein was identified by antisera for the mammalian P170 multidrug resistance protein as a 130 kDa molecule, Binding studies were performed with H-3-vincristine (H-3-VCR) and membrane vesicles ; this process is ATP-dependent and inhibited by verapamil, which is known to reverse the multidrug-resistance phenotype in mammalian systems, Accumulation experiments were …
Alloimmune responses between Acropora hemprichi conspecifics: nontransitive patterns of overgrowth and delayed cytotoxicity
Allogeneic interactions between Acropora hemprichi (Ehrenberg) colonies were followed in situ up to 18 mo between 1990 and 1992 in Eilat, Red Sea. We established all 21 possible branch pair combinations among seven large colonies in three to eight replicates each (n=92 pairs), in addition in 14 pairs of grafts within-colonies. All isografts resulted in fusion. Allogeneic interactions revealed a variety of effector mechanisms which involved the appearance of dark blue-purple color in the tissues of interacting branch tips (<1 cm), unilateral overgrowths (1.0 to 1.5 mm mo-1 for 1 to 8 mo), rejections, healings, continuous overgrowths, reversals, repeated rejections and more. Direction of over…