0000000000485941
AUTHOR
F. Marty
Physiological and Structural Changes in Tobacco Leaves Treated with Cryptogein, a Proteinaceous Elicitor fromPhytophthora cryptogea
Cryptogein was applied on the petiole section of excised tobacco leaves. It elicited necroses that can be correlated with histological alterations, such as rapid chloroplast breakdown and a collapse of cells leading to disorganization of the parenchyma tissue. In addition, it induced ethylene production and accumulation of capsidiol. In order to detect an early response, we analyzed the kinetics of chlorophyll fluorescence induction (...)
The Biogenesis of Vacuoles: Insights from Microscopy
Publisher Summary Vacuoles of plant cells were discovered with the early light microscope and, as meant by the etymology of the word, were subsequently defined as the cell space empty of cytoplasmic matter. As usual in experimental science, methodological and instrumental progress has delineated differently the present definitions of vacuoles, which largely depend on the tools and concepts used for their study. This chapter discusses the biogenesis of vacuoles. Biochemistry and physiology of the vacuole have been made possible by the isolation of intact vacuoles. A specific, although yet incomplete, set of soluble and membrane proteins with unique properties have been specifically ascribed …