Efficacy of clove oil and ethanol against Saprolegnia sp. and usability as antifungal agents during incubation of rainbow trout Oncorhynchus mykiss (Walbaum) eggs

Inhibitory concentrations of clove oil and ethanol against growth of Saprolegnia sp. hyphae were screened by a modification of the hemp (Cannabis sativa L.) seed MicroPlate (HeMP) method and their usability as antifungal agents during incubation of rainbow trout Oncorhynchus mykiss eggs was tested. In vitro experiment showed that in continuous static exposure, clove oil at 100 mg L−1 significantly inhibited the growth of Saprolegnia, whereas in bath exposures, clove oil at 500 mg L−1 had no significant effect at any exposure time tested (15, 60 and 240 min), but clove oil at 10 000 mg L−1 significantly inhibited growth at all exposure times. Clove oil and ethanol treatments had no visible e…

Effects of repeated handling, with or without anaesthesia, on feed intake and growth in juvenile rainbow trout, Oncorhynchus mykiss (Walbaum)

The effects of weekly anaesthetization with clove oil and tricaine methanesulphonate (MS-222) on feed intake and growth were examined in juvenile rainbow trout, Oncorhynchus mykiss (Walbaum), held individually. Repeated handling without anaesthetics significantly reduced feed intake and weight gain compared with an unhandled control group during an 8-week experiment. When anaesthetics were used during handling the feed consumption and weight gain were significantly (MS-222) or not significantly (clove oil) higher than in fish handled without anaesthesia. When compared with the unhandled control group, neither of these two anaesthetics had significant effects on feed intake but, in contrast …

The effect of clove oil sedation on oxygen consumption of six temperate-zone fish species

Temperature effects on anaesthesia with clove oil in six temperate-zone fishes

The potential use of clove oil (eugenol) as an anaesthetic for Atlantic salmon Salmo salar, brown trout Salmo trutta, rainbow trout Oncohynchus mykiss, whitefish Coregonus lavaretus, perch Perca fluviatilis and roach Rutilus rutilus was examined at 5, 10, 15 and 20°C using three anaesthetic concentrations (varying from 20 to 200 mg l -1 ) at each temperature. Substantial species differences in sensitivity to clove oil were observed, even amongst congeners, and there may be some disadvantages (slow recovery and possibly mortality) with using clove oil for 0+ year whitefish and at low temperatures for perch and roach.