RNA-Binding Proteins which interact with mRNAs for H1° and H3.3 histone variants

RNA binding proteins in brain cells differentiation

Cell-to-cell communications among brain cells by extracellular vesicles

Kinetics of rat CSD-C2 binding to H3.3 RNA

Cold-shock domain containing protein C2 (CSD-C2; also known as PIPPin) is an RNA-binding protein conserved in the evolution that interacts with the 3’-untranslated region (3’-UTR) of rat H1.0 and H3.3 histone messengers. Biolayer interferometry (BLI) is a technique that measures changes in an interference pattern generated from visible light, reflected from an optical layer, and a biolayer which contains molecules of interest. In this study, we used the BLI methodology in order to analyze and describe the binding properties of CSD-C2 and the mRNA encoding the rat brain histone protein H3.3. Recombinant CSD-C2 was incubated with in vitro transcribed, and biotinylated H3.3 RNA fragments bound…

Analysis of interaction between messenger RNA encoding H3.3 histone variant and pippin protein by biolayer interferometry

Extracellular microvesicles as brain cell-to-cell communication carriers

Identificazione di proteine che interagiscono con il messaggero dell’istone H1° nel cervello di ratto in sviluppo