0000000000511740

AUTHOR

Nicola Volpe

showing 5 related works from this author

Chromosome analysis using different staining techniques and fluorescent in situ hybridization in Cerithium vulgatum (Gastropoda: Cerithiidae)

2002

In the present paper one population of the “large” subtidal mollusc Cerithium vulgatum Bruguiere, 1792 (Gastropoda: Cerithiidae) from the Northwestern coast of Sicily was investigated from a karyological point of view. The chromosome complement was Giemsa stained, conventionally karyotyped in 18 homomorphic chromosome pairs (10 bi-armed and 8 mono-armed), and subsequently analysed using silver, CMA3 and DAPI staining, and fluorescent in situ hybridization (FISH) with three repetitive DNA probes [ribosomal DNA (rDNA), (TTAGGG)n and (GATA)n]. FISH with the rDNA probe consistently mapped major ribosomal sites (18S-28S rDNA) in the terminal region of the short arms of one small sized mono-armed…

education.field_of_studyStaining and LabelingbiologyBase pairPopulationChromosomeGeneral MedicineRibosomal RNAbiology.organism_classificationMolecular biologyChromosomesBivalent (genetics)Species SpecificityMolluscaChromosome regionsGeneticsAnimalseducationCerithium vulgatumRibosomal DNAIn Situ Hybridization FluorescenceHereditas
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Risk factors associated with adverse fetal outcomes in pregnancies affected by Coronavirus disease 2019 (COVID-19): a secondary analysis of the WAPM …

2020

Abstract Objectives To evaluate the strength of association between maternal and pregnancy characteristics and the risk of adverse perinatal outcomes in pregnancies with laboratory confirmed COVID-19. Methods Secondary analysis of a multinational, cohort study on all consecutive pregnant women with laboratory-confirmed COVID-19 from February 1, 2020 to April 30, 2020 from 73 centers from 22 different countries. A confirmed case of COVID-19 was defined as a positive result on real-time reverse-transcriptase-polymerase-chain-reaction (RT-PCR) assay of nasal and pharyngeal swab specimens. The primary outcome was a composite adverse fetal outcome, defined as the presence of either abortion (pre…

COVID-19 VaccineInfectious Disease TransmissionPerinatal DeathAbortionClinical Laboratory TechniqueMiscarriageCohort Studies0302 clinical medicineCOVID-19 TestingPregnancyRisk Factors3123 Gynaecology and paediatricsSecondary analysisPerinatal medicineAbortion SpontaneouMedicineVertical030212 general & internal medicineViralPregnancy Complications Infectiouscoronavirus; perinatal morbidity; perinatal mortality; covid-19Coronavirus; perinatal morbidity; perinatal mortality; Abortion Spontaneous; COVID-19; COVID-19 Testing; COVID-19 Vaccines; Clinical Laboratory Techniques; Cohort Studies; Coronavirus Infections; Female; Gestational Age; Humans; Infant Newborn; Infant Premature; Infectious Disease Transmission Vertical; Pandemics; Pneumonia Viral; Pregnancy; Pregnancy Complications Infectious; Pregnancy Outcome; Reverse Transcriptase Polymerase Chain Reaction; Risk Factors; SARS-CoV-2; Betacoronavirus; Fetal Death; Perinatal Deathperinatal morbidity030219 obstetrics & reproductive medicineObstetricsReverse Transcriptase Polymerase Chain ReactionInfectiousPregnancy OutcomeGestational ageObstetrics and Gynecology3. Good healthSettore MED/40perinatal mortalityGestationFemaleCoronavirus InfectionsInfant PrematureHumanmedicine.medical_specialtyCOVID-19 VaccinesCoronavirus disease 2019 (COVID-19)CoronaviruPneumonia ViralSocio-culturaleGestational AgeIntrauterine deviceCoronavirus; perinatal morbidity; perinatal mortality03 medical and health sciencesBetacoronavirusPARVOVIRUS B19 INFECTIONCoronavirus perinatal morbidity perinatal mortalityHumansAdverse effectPrematurePandemicsFetal DeathPregnancyFetusBetacoronaviruPandemicCoronavirus Infectionbusiness.industryClinical Laboratory TechniquesSARS-CoV-2Risk FactorSpontaneousMORTALITYInfant NewbornAbortionCOVID-19InfantOdds ratioPneumoniamedicine.diseaseNewbornInfectious Disease Transmission VerticalAbortion SpontaneousPregnancy ComplicationsCoronavirusPediatrics Perinatology and Child HealthPregnancy Complications InfectiouCohort StudiebusinessCoronavirus; perinatal mortality; perinatal morbidityJournal of perinatal medicine
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Repetitive DNAs in the slug Milax nigricans: association of ribosomal (18S–28S and 5S rDNA) and (TTAGGG)n telomeric sequences) in the slug M. nigrica…

2003

Spermatocyte chromosomes of the slug Milax nigricans (Mollusca: Gastropoda: Pulmonata) were studied using silver staining (Ag-NOR) and fluorescent in situ hybridization (FISH) with four repetitive DNA probes [18S rDNA, 5S rDNA, (TTAGGG)n and (GATA)n]. Silver impregnation was inadequate to localize the chromosome sites of nucleolus organizer regions (NORs) since no silver dots occurred on the chromosomes at spermatogonial metaphase and a diffuse silver stainability could be observed on the bivalents at metaphase-I. Unlike silver staining, single-colour rDNA FISH consistently mapped major ribosomal sites (18S-28S rDNA) on two small-sized chromosomes in spermatogonial cells and on the correspo…

MaleSilverNucleolusGeneral Physics and AstronomyBiologyDNA RibosomalBivalent (genetics)Silver stainSpermatocytesStructural BiologyRNA Ribosomal 28SNucleolus Organizer RegionRNA Ribosomal 18SAnimalsGeneral Materials ScienceRepeated sequenceMetaphaseIn Situ Hybridization FluorescenceRepetitive Sequences Nucleic AcidGeneticsBase SequenceStaining and LabelingRNA Ribosomal 5SChromosomeDNACell BiologyTelomereRibosomal RNAMolecular biologyMolluscaNucleolus organizer regionMicron
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"KARYOTYPE, BANDING AND rDNA FISH IN THE SCARAB BEETLE ANOPLOTRUPES STERCOROSUS (COLEOPTERA SCARABAEOIDEA: GEOTRUPIDAE). DESCRIPTION AND COMPARATIVE …

2004

Six specimens of Anoplotrupes stercorosus (Coleoptera Scarabaeoidea: Geotrupidae) were analysed using conventional staining, banding techniques and fluorescent in situ hybridization with a ribosomal probe (rDNA FISH). Detailed karyotype description was also joined to a comparative analysis between present data and those previously reported for Thorectes intermedius [Chromosome Res. 7 (1999) 1]. The two species, both belonging to the tribe Geotrupini, show the same modal number but different autosomal morphology which is in contrast with the high chromosome stability argued for Geotrupinae during the last three decades. Moreover, a detailed comparison reveals the occurrence of a plesiomorphi…

Malemedicine.medical_specialtyGeneral Physics and AstronomyZoologyScarabaeoideaTribe (biology)DNA RibosomalStructural BiologyGeotrupidaemedicine18S rDNA FISHAnimalsGeneral Materials ScienceIn Situ Hybridization FluorescencebiologyPhylogenetic treecytogeneticCytogeneticsKaryotypeCell BiologyRibosomal RNAbiology.organism_classificationAnoplotrupes stercorosusChromosome BandingColeopteraKaryotypingFemaleColeoptera Scarabaeoidea Geotrupidae
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Evidence for male XO sex-chromosome system in Pentodon bidens punctatum (Coleoptera Scarabaeoidea: Scarabaeidae) with X-linked 18S-28S rDNA clusters

2004

In scarab beetle species of the genus Pentodon, the lack of analysis of sex chromosomes in females along with the poor characterization of sex chromosomes in the males, prevented all previous investigations from conclusively stating sex determination system. In this study, somatic chromosomes from females and spermatogonial chromosomes from males of Pentodon bidens punctatum (Coleoptera: Scarabaeoidea: Scarabaeidae) from Sicily have been analyzed using non-differential Giemsa staining. Two modal numbers of chromosomes were obtained: 2n = 20 and 19 in females and males, respectively. This finding along with other karyological characteristics such as the occurrence of one unpaired, heterotypi…

MaleGeneticsSex ChromosomesbiologyHeterochromatinZoologyChromosomeKaryotypeGeneral MedicineScarabaeoideabiology.organism_classificationChromosome BandingColeopteraHeterochromatinKaryotypingGeneticsSex-determination systemAnimalsConstitutive heterochromatinFemaleMolecular BiologyRibosomal DNAIn Situ Hybridization FluorescenceX chromosomeGenes & Genetic Systems
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