0000000000515362
AUTHOR
Lelde Grantina
Monitoring seasonal changes in microbial populations of spruce forest soil of the Northern Temperate Zone
Soil microbial populations in the Northern Temperate Zone have been poorly studied in comparison with extreme environments. The aim of the work was to study the seasonal changes in the microbial populations of spruce forest soil of the Northern Temperate Zone using classical methods of microbiology and molecular biology. Upper horizons in two Picea abies stands on sod- podzolic and illuvial humus podzol soil were analysed. Sampling was done monthly over a period of twelve months (May 2009-April 2010). Microbial communities in both experimental plots showed different responses to the analysed environmental factors. In the sod-podzolic soil only the fungal DNA amount was significantly higher …
Growth and Antagonism of <i>Trichoderma</i> spp. and Conifer Pathogen <i>Heterobasidion annosum</i> s.l. <i>in Vitro</i> at Different Temperatures
Variations in the radial growth rate of 24 isolates belonging to ten species of Trichoderma, three isolates of conifer pathogen Heterobasidion annosum s.s. and four isolates of H. parviporum were evaluated by incubation on a solid malt extract medium at a temperature of 4℃, 15℃ and 21℃. Trichoderma antagonism against Heterobasidion was investigated in dual culture in vitro. The slowest rate of growth was referable to all seven strains of Heterobasidion spp. All Heterobasidion spp. strains were overgrown by 63% of Trichoderma spp. strains after two weeks at 21℃ and by 33% of strains at 15℃. 21% of Trichoderma strains did not grow and only four strains belonging to T. koningii, T. viride and …