0000000000519209

AUTHOR

Mario Mastrangelo

showing 3 related works from this author

A taxonomic survey of lactic acid bacteria isolated from wheat (Triticum durum) kernels and non-conventional flours

2007

In order to explore the correspondence between raw material- and mature sourdough-lactic acid bacterial (LAB) communities, 59 Italian wheat (Triticum durum) grain samples, one bran and six non-conventional flour samples were analyzed through a culture-dependent approach. The highest cell count by an agar medium specific for LAB was 2.16 log CFU/g. From about 2300 presumptive LAB (Gram-positive and catalase-negative) colonies collected, a total of 356 isolates were subjected to identification by a genetic polyphasic strategy consisting of RAPD-PCR analysis, partial 16S rRNA gene sequencing, species-specific and multiplex PCRs. The isolates were recognized as 137 strains belonging to Aerococc…

DNA BacterialDietary FiberLactococcusEnterococcus mundtiiFlourMolecular Sequence Dataculture-dependent niethods genetic polyphasic approach lactic acid bacteria non-conventional flours sourdough Triticum durumColony Count MicrobialGram-Positive BacteriaApplied Microbiology and BiotechnologyMicrobiologyDNA RibosomalMicrobiologyLactobacillusRNA Ribosomal 16SSequence Homology Nucleic Acidmetodi coltura-dipendenti approccio polifasico genetico impasti acidiFood scienceLactic AcidEcology Evolution Behavior and SystematicsPhylogenyTriticumgenetic polyphasic approachsourdoughbiologyfood and beveragesGenes rRNASequence Analysis DNAbiology.organism_classification16S ribosomal RNACatalaseDNA FingerprintingRandom Amplified Polymorphic DNA Techniquelactic acid bacteriaRNA BacterialEnterococcusItalyTriticum durumAerococcusPediococcusEdible Grainnon-conventional floursculture-dependent niethodsEnterococcus faecium
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Diagnostic Targeted Resequencing in 349 Patients with Drug-Resistant Pediatric Epilepsies Identifies Causative Mutations in 30 Different Genes

2017

Targeted resequencing gene panels are used in the diagnostic setting to identify gene defects in epilepsy. We performed targeted resequencing using a 30-genes panel and a 95-genes panel in 349 patients with drug-resistant epilepsies beginning in the first years of life. We identified 71 pathogenic variants, 42 of which novel, in 30 genes, corresponding to 20.3% of the probands. In 66% of mutation positive patients seizures onset occurred before age 6 months. The 95-genes panel allowed a genetic diagnosis in 22 (6.3%) patients that would have otherwise been missed using the 30-gene panel. About 50% of mutations were identified in genes coding for sodium and potassium channel components. SCN2…

0301 basic medicineProbandMaleCDKL5Drug Resistancemedicine.disease_causeBioinformaticsEpilepsyAnticonvulsantSTXBP1Age of OnsetChildGenetics (clinical)AlleleMutationepilepsy; next-generation sequencing; gene panel; mutationPhenotypeMagnetic Resonance ImagingSettore MED/39 - Neuropsichiatria Infantile3. Good healthPhenotypeChild PreschoolAnticonvulsantsFemaleSequence AnalysisHumanAdolescentGenotypeGenetic Association StudieBiologyMECP203 medical and health sciencesGeneticgene panelGeneticsmedicineHumansGenetic Predisposition to DiseasePreschoolGeneAllelesGenetic Association StudiesGene Expression ProfilingInfant NewbornComputational BiologyInfantMolecular Sequence AnnotationDNASequence Analysis DNANewbornmedicine.disease030104 developmental biologyepilepsynext-generation sequencingmutation
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Identification of subdominant sourdough lactic acid bacteria and their evolution during laboratory-scale fermentations

2007

Abstract Presumptive lactic acid bacterial cocci were found in six sourdoughs (out of 20) from the Abruzzo region (central Italy) and subjected to phenotypic and genotypic characterization. A total of 21 isolates, recognized as seven strains by randomly amplified polymorphic DNA (RAPD)–polymerase chain reaction (PCR) typing, were identified by a polyphasic approach, consisting of 16S rRNA gene sequencing, multiplex PCR assays and physiological features, as Enterococcus faecium and Pediococcus pentosaceus. Four strains belonging to those species and previously isolated from wheat kernels were inoculated in sterile flour to verify their capacity to grow in sourdough environment. Doughs with s…

GenotypeColony Count MicrobialLactobacillus sanfranciscensisMicrobiologyMicrobiologychemistry.chemical_compoundSpecies SpecificityRNA Ribosomal 16Sco-fermentazioni batteri lattici sottodominantiMultiplex polymerase chain reactionPediococcusTypingPhylogenybiologyfood and beveragesBreadHydrogen-Ion Concentrationbiology.organism_classificationRandom Amplified Polymorphic DNA TechniqueRAPDLactic acidLactobacillusRNA BacterialPhenotypechemistryFermentationFood MicrobiologyFermentationEnterococcusBacteriaFood ScienceEnterococcus faeciumFood Microbiology
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