0000000000522570
AUTHOR
P. Mantovani
4-Aminopyridine (4AP) Enhances Acetylcholine Output from the Rat Cerebral Cortex in vivo
Publisher Summary This chapter discusses a study to analyze the effect of 4-aminopyridine (4AP) administration on cortical acetylcholine (ACh) output. The study was done on adult male Wistar rats under urethane or pentobarbital anesthesia. A small Perspex cylinder filled with eserinized Ringer solution was applied on the exposed cerebral cortex. The solution in the collecting cylinder was removed every 10 min and its ACh content was determined by bioassay on the dorsal muscle of the leech. In urethane anaesthetized rats, the control ACh output was 0.98 ± 0.16 ng/min/cm2 and the administration of 4AP (3 mg/kg i.p.) was followed by a rapid increase in ACh output lasting at least 40 min. The i…
Prove nazionali. I risultati del 46o anno di sperimentazione. Duro, i consigli varietali
In 31 campi dei principali areali di coltivazione italiani sono stati valutati 38 genotipi, di cui 12 al primo anno di prova. La cultivar mediamente più produttiva è ri sultata LG anubis, al primo anno, con indice 108 e medie superate in 25 prove su 31 totali (81% dei campi), caratterizzata da peso ettolitrico e tenore proteico leggermente inferiori alle medie (-0,1 e -0,4 punti, rispettivamente).
EFFECTS OF 4-AMINOPYRIDINE ON ACETYLCHOLINE OUTPUT FROM THE CEREBRAL CORTEX OF THE RAT in vivo
Abstract 1 The effects of 4-aminopyridine (4AP) on the output of acetylcholine (ACh) from the cerebral cortex were investigated in unanaesthetized freely moving rats and in anaesthetized rats by means of the `cup technique'. ACh was determined by bioassay on the dorsal muscle of the leech. 2 In unanaesthetized rats intraperitoneal injection of 4AP (3 mg/kg) had no effect on the cortical output of ACh. 3 After injection of morphine (10 mg/kg s.c.), which depressed the spontaneous output of ACh, 4AP increased the cortical output to a level significantly higher than that determined before morphine injection. 4 In rats anaesthetized with either urethane or pentobarbitone, drugs known to decreas…