Identity and relationships of Sempervivum tectorum (Crassulaceae) in the Rhine Gorge area
Sempervivum tectorum (Crassulaceae), an orophyte widespread in the European high mountains, also grows in rocky habitats of the Rhine Gorge area (Upper Middle Rhine, Mosel and Ahr river valleys). On the background of its long history of cultivation, it is unclear whether S. tectorum is native or naturalized in the Rhine Gorge area. Using 52 accessions of S. tectorum from across its geographical range (except SE Europe) as well as 15 samples of S. calcareum and S. marmoreum in our final sample, we conducted a genotyping-by-sequencing analysis. The genetic data were used for the identification of genetic groups and for the reconstruction of phylogenetic relationships. We found that the materi…
Hybridization, ecogeographical displacement and the emergence of new lineages – A genotyping‐by‐sequencing and ecological niche and species distribution modelling study of Sempervivum tectorum L. (Houseleek)
Ecogeographical displacement of homoploid hybrid lineages from their parents is well documented and considered an important mechanism to achieve reproductive isolation. In this study, we investigated the origin of the flowering plant species Sempervivum tectorum in the Massif Central (France) through homoploid hybridization between lineages of the species from the Rhine Gorge area (Germany) and the Pyrenees (France). We used genotyping-by-sequencing genetic data as evidence for the hybrid origin of the Massif Central lineage, and WorldClim climatic data and soil pH and soil temperature data collected by us for ecological niche and species distribution modelling. We could show that the Massi…
Characterisation of microsatellite and SNP markers from Miseq and genotyping-by-sequencing data among parapatric Urophora cardui (Tephritidae) populations
Phylogeographic analyses of the gall flyUrophora carduihave in earlier studies based on allozymes and mtDNA identified small-scale, parapatrically diverged populations within an expanding Western Palearctic population. However, the low polymorphism of these markers prohibited an accurate delimitation of the evolutionary origin of the parapatric divergence.Urophora carduifrom the Western Palearctic have been introduced into Canada as biological control agents of the host plantCirsium arvense. Here, we characterise 12 microsatellite loci with hexa-, penta- and tetra-nucleotide repeat motifs and report a genotyping-by-sequencing SNP protocol. We test the markers for genetic variation among thr…