0000000000525896

AUTHOR

Toraj Babaei

showing 2 related works from this author

Detection and quantification of Streptococcus pneumoniae from Iranian patients with pneumonia and individual carriers by real time PCR

2011

The aim of this study was to develop a real time polymerase chain reaction (PCR) for quantitative detection of Streptococcus pneumoniae from clinical respiratory specimens. Initially, 184 respiratory specimens from patients with community acquired pneumonia (CAP) (n = 129) and 55 cases with hospital associated pneumonia (HAP) were bacteriologically investigated. To check the colonization status among the healthy individuals, 32 preschool and 31 adults were screened in parallel. All specimens were cultured on selective culture media to isolate S. pneumoniae, Legionella spp. and Mycoplasma spp. A 166 bp fragment corresponding to cbp A gene of S. pneumoniae was amplified from clinical specimen…

Mycoplasma pneumoniaeSettore MED/07 - Microbiologia E Microbiologia ClinicabiologyLegionellaStreptococcus pneumoniae community acquired pneumonia (CAP) real time polymerase chain reaction (PCR) choline binding protein A (cbp AMycoplasmamedicine.disease_causemedicine.diseasebiology.organism_classificationApplied Microbiology and BiotechnologyVirologyrespiratory tract diseasesMicrobiologyPneumoniaCommunity-acquired pneumoniaStreptococcus pneumoniae community acquired pneumonia (CAP) real time polymerase chain reaction (PCR) choline binding protein A (cbp A).Chlamydophila pneumoniaeStreptococcus pneumoniaeGeneticsmedicineTaqManAgronomy and Crop ScienceMolecular BiologyBiotechnology
researchProduct

Single tube real time PCR for detection of Streptococcus pneumoniae, Mycoplasma pneumoniae, Chlamydophila pneumoniae and Legionella pneumophila from …

2012

We designed a multiplex real time PCR for rapid, sensitive and specific detection of Streptococcus pneumoniae, Legionella pneumophila, Chlamydophila pneumoniae and Mycoplasma pneumoniae. The study cases consisted of 129 patients with community acquired pneumonia (CAP). Bacteriological techniques were implemented for detection of the cultivable organisms. DNA were extracted from sputa, throat swabs, bronchoalveolar lavages and tracheal aspirates and used as templates in real time PCR. The primers and probes were designed for cbpA (S. pneumoniae), p1adhesin (M. pneumoniae), mip (L. pneumophila) and ompA (C. pneumoniae). After optimization of real time PCR for every organism, the experiments w…

Settore MED/07 - Microbiologia E Microbiologia ClinicaMycoplasma pneumoniaemedicine.disease_causeReal-Time Polymerase Chain ReactionLegionella pneumophilaSensitivity and SpecificityMicrobiologyLegionella pneumophilaCommunity-acquired pneumoniacommunity acquired pneumonia CAP real time PCR Streptococcus pneumonia Legionella pneumophila Chlamydophila pneumonia Mycoplasma pneumoniaeStreptococcus pneumoniaeMultiplex polymerase chain reactionmedicinePneumonia BacterialHumansMultiplexGeneral Immunology and MicrobiologybiologyBacteriaGeneral MedicineChlamydophila pneumoniaebiology.organism_classificationmedicine.diseaseVirologyrespiratory tract diseasesMycoplasma pneumoniaeCommunity-Acquired InfectionsReal-time polymerase chain reactionStreptococcus pneumoniaeChlamydophila pneumoniaeMultiplex Polymerase Chain ReactionActa microbiologica et immunologica Hungarica
researchProduct