Evaluation of chloroplast genome annotation tools and application to analysis of the evolution of coffee species.
International audience; Chloroplast sequences are widely used for phylogenetic analysis due to their high degree of conservation in plants. Whole chloroplast genomes can now be readily obtained for plant species using new sequencing methods, giving invaluable data for plant evolution However new annotation methods are required for the efficient analysis of this data to deliver high quality phylogenetic analyses. In this study, the two main tools for chloroplast genome annotation were compared. More consistent detection and annotation of genes were produced with GeSeq when compared to the currently used Dogma. This suggests that the annotation of most of the previously annotated chloroplast …
The defensive secretion of Eurycotis floridana (Dictyoptera, Blattidae, Polyzosteriinae): chemical identification and evidence of an alarm function
0965-1748 doi: DOI: 10.1016/S0965-1748(97)00033-7; The defensive secretion of the cockroach Eurycotis floridana was believed to contain only (E)-2-hexenal. However, we have shown it consists of 40 components, of which 30 were tentatively identified. (E)-2-Hexenal, (E)-2-hexenol and (E)-2-hexenoic acid represented approximately 98% of the organic phase. The other 2% included 10 aldehydes, 10 alcohols, four acids, two lactones and one ether. Four compounds are novel insect exudates: 3-ethoxyhexanal, 3-hydroxyhexanal, [(E)-1-pentenyl]-4-propyl-1,3-dioxane and 3-[(E)-2-hexenoxyl-hexanal. In addition to its well-known allomonal function, we have demonstrated that the defensive secretion also act…
Quantitative determination of potent flavor compounds in Burgundy Pinot noir wines using a stable isotope dilution assay
A specific experimental procedure suitable for the quantification of four esters recently identified in a wine of Vitis vinifera cv. Pinot Noir, ethyl dihydrocinnamate (A), ethyl cinnamate (B), methyl anthranilate (C), and ethyl anthranilate (D), was developed and applied to 33 Burgundy wines (calculated on three replicates). The method, involving a stable isotope dilution assay, allows the determination of concentrations from 0.05 μg L-1, with a repeatability better than 10%. The mean, maximum, and minimum amounts found for the four esters were as follows (in μg L-1): (A) 1.6, 3.2, 0.8; (B) 0.8, 1.6, 0.5; (C) 0.2, 0.6, 0.06; (D) 2.4, 4.8, 0.6. Differences between wines, according to their…