0000000000527384
AUTHOR
F. Ales
Cancer cell(s) cycle sequencing reveals universal mechanisms of apoptosis
In this paper, cell cycle in higher eukaryotes and their molecular networks signals both in G 1/S and G2/M transitions are replicated in silico. Biochemical kinetics, converted into a set of differential equations, and system control theory are employed to design multi-nested digital layers to simulate protein-to-protein activation and inhibition for cell cycle dynamics in the presence of damaged genomes. Sequencing and controlling the digital process of four micro-scale species networks (p53/Mdm2/DNA damage, p21mRNA/cyclin-CDK complex, CDK/CDC25/wee1/ SKP2/APC/CKI and apoptosis target genes system) not only allows the comprehension of the mechanisms of these molecule interactions but paves…
The microbial community of sub-surface sediment of a chronically contaminated SIC
Hydrocarbons (HC), especially high molecular weight HC, are trapped in the sediments for a long time, making the benthic system a permanent pollution source, with several effects on the biota. Sediment bacterial communities play a significant role in the degradation of contaminants, both under aerobic and anaerobic conditions. In this work we focused on the bacterial communities of sediments (5-10 cm below surface), of a Site of Community Importance, the Priolo Bay, in Central Mediterranean Sea. The bay is situated in proximity to the Augusta Harbour, affected for decades by pollution from industrial and petrochemical plants. The microbial communities of sediments from six stations on two t…
On Cancer Cell Cycle and Universal Apoptosis Parameters Signaling Unravelled In Silico
Here, cell cycle in higher eukaryotes and their molecular networks signals both in G1/S and G2/M transitions are in silico replicated. Systems control theory is employed to design multi-nestled digital layers to simulate protein-toprotein activation and inhibition in the cancer cell cycle dynamics in presence of damaged genome. Sequencing and controlling the digital process of four micro-scale species networks (p53/Mdm2/DNA damage; p21mRNA/cyclin-CDK complex; CDK/CDC25/wee1/SKP2/APC/CKI and apoptosis target genes system) paved the way for unravelling the participants and their by-products having the task to execute (or not) cell death. The results of the proposed cell digital multi-layers g…