0000000000530696

AUTHOR

Uwe Wendling

showing 3 related works from this author

An MHC class II-expressing T cell clone presenting conventional antigen lacks the ability to present bacterial superantigen.

1995

We have analyzed the response of rat T cells to myelin basic protein (MBP) and the bacterial superantigen, staphylococcal enterotoxin E (SEE). Rat T cells reactive with MBP can respond to SEE presented by spleen cells but not to SEE presented by LOA, a rat T cell clone that expresses both I-A and I-E MHC class II molecules, even though LOA is much more efficient than splenic APC in the presentation of MBP. The inability of LOA to present superantigen is not due to a structural difference in MHC II molecules between LOA and the splenic APC or to differential expression of major accessory/adhesion molecules, including CD2, CD5, CD4 and CD44, on LOA. The non-responsiveness of SEE/LOA-induced T…

Staphylococcus aureusT cellT-LymphocytesImmunologyAntigen-Presenting CellsEnterotoxinsInterferon-gammaAntigenparasitic diseasesMHC class ImedicineImmunology and AllergyCytotoxic T cellAnimalsClonal AnergyMHC class IIAntigens BacterialSuperantigensbiologyAntigen processingChemistryHistocompatibility Antigens Class IIMyelin Basic ProteinGeneral MedicineMHC restrictionClone CellsRatsmedicine.anatomical_structureRats Inbred LewImmunologybiology.proteinCD8International immunology
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Full length cDNA of rat RT1.DMa and RT1.DMb and expression of RT1.DM genes in dendritic and Langerhans cells.

1997

MHC encoded DM heterodimers and classical MHC class II complexes meet in an endosomal/lysosomal compartment where DM heterodimers support peptide loading of MHC class II. Studies on peptide loading of rat class II and on peptide persistence in cells of the dendritic lineage prompted us to establish full length cDNA clones coding for the subunits alpha and beta of rat DM molecules as well as a mAb directed against the luminal moiety of the beta subunit. Here we describe the establishment of the first full length cDNA clones of rat RT1.DMa and RT1.DMb. The mode of expression of RT1.DM at the transcript level in bone marrow culture-derived dendritic cells, in Langerhans cells and in a number o…

Maleendocrine systemDNA ComplementaryTranscription Geneticmedicine.drug_classClinical BiochemistryBlotting WesternGenes MHC Class IIMolecular Sequence DataGene ExpressionBone Marrow CellsMonoclonal antibodyMajor histocompatibility complexBiochemistryIslets of LangerhansHistocompatibility AntigensGene expressionmedicineAnimalsAmino Acid SequenceCloning MolecularMolecular BiologyPeptide sequenceCells CulturedMHC class IIbiologyBase SequenceChemistryAntibodies MonoclonalDendritic CellsBlotting NorthernMolecular biologyRatsBlotRats Inbred Lewbiology.proteinBeta proteinAntibodyBiological chemistry
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Complete coding nucleotide sequence of cDNA for the class II RT1.B beta I chain of the Lewis rat.

1991

We have established the first full length cDNA clone for the beta light chain of the MHC class II alpha, beta heterodimer (isotype RT1.B) of the rat. Clone pLR beta 118 was obtained from a self-primed lambda gt10 cDNA library of IFN-tau treated bone marrow-derived macrophages of the Lewis rat. Subcloning of pLR beta 118 into a transcription vector with subsequent in vitro transcription and translation using the reticulocyte lysate system in the presence of microsomes followed by immunoprecipitation with mAb OX6 and two-dimensional gel electrophoresis revealed the intact RT1.B beta I-chain.

ImmunoprecipitationMolecular Sequence DataBiophysicsBone Marrow CellsBiologyImmunoglobulin light chainTransfectionBiochemistryReticulocyteStructural BiologyTranscription (biology)Complementary DNAHistocompatibility AntigensGeneticsmedicineAnimalsElectrophoresis Gel Two-DimensionalAmino Acid SequenceCells CulturedBase SequencecDNA libraryMacrophagesNucleic acid sequenceDNAExonsMolecular biologyRatsmedicine.anatomical_structureSubcloningRats Inbred LewBiochimica et biophysica acta
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