0000000000535420
AUTHOR
Eliane Cases
Effect of high methoxyl pectin on pea protein in aqueous solution and at oil/water interface
International audience; The effect of the addition of high methoxyl pectin on the stability of pea protein isolate emulsions was investigated. Except for low pectin concentrations at acidic pHs where bridging flocculation occurred the addition of pectin improved emulsion stability to pH changes and depletion flocculation induced by maltodextrin addition. The mechanism of pectin induced stability was probed by measuring protein-pectin complex formation in solution, zeta potential of the emulsions droplets and the change in surface viscoelasticity on pectin addition. The phase diagrams of pectin-pea protein isolate in solution and pectin-pea protein-stabilized emulsions were established based…
Impact of processing conditions on functionality of powders prepared from mixed protein system
International audience; The projection of a global human population of around 9.5 billion by 2050 indicates that the demand for animal protein will double during this period. It seems therefore essential in regard to sustainability and food security to search for alternative protein sources that show nutritional and functional quality close to animal protein ones. In that respect, there is a growing interest in utilizing plant proteins as partial replacers of animal proteins in food. The reintroduction of plant proteins into our diet is a solution already envisaged by some vegetarian or flexitarian diets. In order to broaden this practice, one strategy is the use of protein mixes as an ingr…
VEggInInteractions protéines végétales –protéines de blanc d’œuf pour le développement d’ingrédients fonctionnalisés
compréhension des interactions entre protéines végétales et protéines de blanc d’œuf pour permettre l’amélioration de la « techno-fonctionnalité » des mélanges, notamment pour la stabilisation des mousses et émulsions, l’optimisation de ces fonctionnalités et l’identification des conditions permettant de limiter leur sensibilité aux traitements thermiques.
The foaming properties of camel and bovine whey: The impact of pH and heat treatment
International audience; he effect of heat treatment (70 degrees C or 90 degrees C for 30 min) on the foaming and interfacial properties of acid and sweet whey obtained from bovine and camel fresh milk was examined. The maximum foamability and foam stability were observed for acid whey when compared to sweet whey for both milks, with higher values for the camel whey. This behavior for acid whey was explained by the proximity of the pI of whey protein (4.9-5.2), where proteins were found to carry the lowest negative charge as confirmed by the zeta potential measurements. Interfacial properties of acid camel whey and acid bovine whey were preserved at air water interface even after a heat trea…
Improvement of the techno-functional properties of pea proteins by microfluidization
The use of pea (Pisum sativum (L.)) proteins in the food industry is still limited despite their good environmental sustainability, heath-oriented composition, reliable origin and stable price. However, one of the most important limitations is their low solubility which determines a number of their techno-functional properties. Microfluidization is a non-thermal emerging technology that may modify the structure and the techno-functional properties of pea proteins. Microfluidization combines high shear forces due to the stream speed and direction; impact forces from collisions with the walls and with the fluid itself; and turbulence inside the chamber. The objective of this work was to evalu…
Effect of apolar phase dielectric constant on interfacial properties of β-lactoglobulin (dielectric constant and interfacial properties of β-lactoglobulin)
International audience; In this work, we hypothesized that the difference in the dielectric constant value existing between two non-miscible phases such as oil and water could be partially responsible for both protein unfolding and reorganization of the protein structure at the interface. So, we replaced the oil phase, whose dielectric constant value is 2, by organic solvents chosen for both their non-miscibility with water and their range of dielectric constant values higher (range 6.1-7.2) than that of oil. Using a dynamic drop tensiometer, we studied both surface activity and viscoelastic properties of the milk protein at various interfaces. The aqueous phase (pH7; 25°C) contained the co…
Utilisation of pectin coating to enhance spray-dry stability of pea protein-stabilised oil-in-water emulsions
International audience; In this study, development of pea (Pisum sativum) protein stabilised dry and reconstituted emulsions is presented. Dry emulsions were prepared by spray-drying liquid emulsions in a laboratory spray-dryer. The effect of drying on the physical stability of oil-in-water emulsions containing pea protein-coated and pea protein/pectin-coated oil droplets has been studied. Oil-in-water emulsions (5 wt.% Miglyol 812 N, 0.25 wt.% pea protein, 11% maltodextrin, pH 2.4) were prepared that contained 0 (primary emulsion) or 0.2 wt.% pectin (secondary emulsion). The emulsions were then subjected to spray-drying and reconstitution (pH 2.4). The stability of the emulsions to dry pro…
MOOC : "ProtéiNNOV : Protéines végétales et Innovation"
La prise de conscience d'une raréfaction des ressources en protéines animales dans les décennies à venir et la nécessité de produire plus durablement ces ressources alimentaires nous conduit à repenser aux autres sources de protéines, notamment les protéines d'origine végétale. Parmi les sources de protéines végétales, la plus employée comme substitut des protéines animales est le soja, mais le soja n'est pas la seule source de protéines végétales. Il est donc opportun de faire le point sur les autres sources de protéines végétales et sur leur utilisation. Le développement de nouveaux produits alimentaires riches en protéines végétales passe par la connaissance des atouts tant technologique…
Effect of iron chelates on oil–water interface, stabilized by milk proteins: The role of phosphate groups and pH. Prediction of iron transfer from aqueous phase toward fat globule surface by changes of interfacial properties
International audience; Iron incorporated into food systems induces oxidation and precipitation. The consequences are reduced bioavailability and a functional modification of other food components such as proteins. The iron-chelates such as ferrous bisglycinate represent a possibility to avoid side effects, since the iron is protected. The aim of this study is to investigate the effects of iron-chelates compounds on the properties of an oil/water interface stabilized by caseinate or β-lacotoglobulin, under environmental conditions at 20 °C. Analyses were performed using dynamic drop tensiometry during 5000 s. The aqueous bulk phase is an imidazole/acetate buffer (0.1 M), containing 0.4 × 10…