0000000000537869

AUTHOR

Martina Baltkalne

showing 2 related works from this author

Discriminative features of type I and type III secreted proteins from Gram-negative bacteria

2006

AbstractThe amino acid composition of sequences and structural attributes (α-helices, β-sheets) of C-and N-terminal fragments (50 amino acids) were compared to annotated (SWISS-PROT/ TrEMBL) type I (20 sequences) and type III (22 sequences) secreted proteins of Gram-negative bacteria.The discriminant analysis together with the stepwise forward and backward selection of variables revealed the frequencies of the residues Arg, Glu, Gly, Ile, Met, Pro, Ser, Tyr, Val as a set of strong (1-P < 0.001) predictor variables to discriminate between the sequences of type I and type III secreted proteins with a cross-validated accuracy of 98.6–100 %. The internal and external validity of discriminant…

chemistry.chemical_classificationGram-negative bacteriaGeneral Immunology and MicrobiologybiologyQH301-705.5General Neurosciencediscriminant analysisbiology.organism_classificationLinear discriminant analysisgram-negative bacteriaGeneral Biochemistry Genetics and Molecular Biologyamino acid sequenceAmino acidSecretory proteinBiochemistrychemistryprotein secretionSecretionBiology (General)General Agricultural and Biological SciencesPeptide sequenceBacteriaGramOpen Life Sciences
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The effect of amphiphilic compounds on the secretion of levansucrase by Zymomonas mobilis

2005

Abstract The effect of some aliphatic (n-butanol to n-hexadecanol) and aromatic (benzyl and phenethyl alcohols), anesthetics (procaine) and surfactants (Tween 20 to Tween 80) on the secretion of levansucrase by the levan-producing strain of Gram-negative ethanologenic bacteria Zymomonas mobilis 113S were examined in this study. During incubation of Z. mobilis cells with sucrose (10 mM) a decrease of the levansucrase activity was observed in the presence of these amphiphilic compounds concomitantly with an increase of a total amount of protein in the medium. Since none of the compounds under study had any effect on enzyme activity in vitro observed structure- and concentration-dependent rela…

biologyChemiosmosisProtonophoreLevansucrase activityATPaseLevansucraseBioengineeringbiology.organism_classificationApplied Microbiology and BiotechnologyBiochemistryZymomonas mobilischemistry.chemical_compoundBiochemistrychemistrybiology.proteinSodium azideSecretionProcess Biochemistry
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