0000000000539866

AUTHOR

J. García De Lomas

showing 2 related works from this author

Sequential variation in genomic RNA patterns of human rotaviruses isolated from infantile gastroenteritis

1986

The incidence and RNA electrophoretypes of rotaviral isolates obtained from infants and young children with acute gastroenteritis were studied from October, 1985 through April, 1986. Analysis of the viral RNA was carried out by Polyacrylamide gel electrophoresis followed by silver staining. Fourteen electrophoretypes were identified. A single dominant electrophoretype was found during the first months of the rotavirus seasonal outbreak. In contrast, a large variety of RNA patterns were observed during the latter portion of the study period. Subgrouping of rotavirus isolates by a double-sandwich enzyme-linked immunosorbent assay using monoclonal-detecting antibodies showed that all strains b…

Gel electrophoresisMolecular epidemiologyOutbreakRNAReoviridaeGeneral MedicineBiologyEnfant Epidémiologie moléculaire Electrophorétypes Profils de l’ARN génomique Variation séquentiellemedicine.disease_causebiology.organism_classificationVirologyArticleVirusMicrobiologyMolecular epidemiology Genomic RNA patterns Sequential variation Electrophoretypes ChildrenSilver stainRotavirus Gastroentérite ARNRotavirus RNA GastroenteritisRotavirusmedicineAnnales de l'Institut Pasteur / Virologie
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Wall mannoproteins in cells from colonial phenotypic variants of Candida albicans.

1990

Candida albicans ATCC 26555 switched at high frequency (10(-1) to 10(-3)) between several phenotypes identified by colony morphology on a defined mineral amino-acid-containing agar medium supplemented with arginine and zinc (LAZ medium). When cells taken from colonies exhibiting distinct morphologies were plated directly onto LAZ agar, spontaneous conversion to all the variant phenotypes occurred at combined frequencies of 2.1 x 10(-1) to 9.5 x 10(-3). However, when cells taken from the different colonial phenotypes were plated directly onto an undefined medium (yeast extract/peptone/dextrose; YPD medium), or first incubated in liquid YPD medium and then cloned on YPD agar, all colonies obs…

food.ingredientHydrolasesPhenotypic switchingMicrobiologyMicrobiologyAgar plateCell wallFungal Proteinschemistry.chemical_compoundfoodCell WallCandida albicansConcanavalin AAgarCandida albicansAntiserumGrowth mediumMembrane GlycoproteinsbiologyTemperaturebiology.organism_classificationYeastCulture MediaPhenotypechemistryElectrophoresis Polyacrylamide GelJournal of general microbiology
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