0000000000540912
AUTHOR
G. Giammona
NEW BIODEGRADABLE HYDROGELS DESIGNED FOR COLONIC DRUG DELIVERY: IN VITRO RELEASE OF THE PEPTIDES GLUTATHIONE AND OXYTOCIN
MICROFLUIDIC PROCEDURE FOR FABRICATION OF HYALURONIC ACID BASED BIOMATERIALS
UPTAKE OF SILICA COVERED QUANTUM DOTS ON LIVING CELLS: LONG TERM VITALITY AND MORPHOLOGY STUDY ON HYALURONIC ACID BIOMATERIALS
In this work we prepared colloidal CdSe-CdS core-shell nanocrystal emitting @625 nm and, by a micro-emulsion procedure of water in oil inverse micelle, we encapsulated a single dot inside each silica sphere. In this way the nanoparticles became biocompatible, are simply to prepare and could be used also in a water based environment. Moreover, the silica shield is transparent to the wavelengths of interest.
AMPHIPHILIC GRAFT COPOLYMER OF HYALURONIC ACID AND POLYLACTIC ACID FOR PULMONARY DELIVERY OF AMPHOTERICIN B
A New Hyaluronic Acid Derivative Obtained from Atom Transfer Radical Polymerization as a siRNA Vector for CD44 Receptor Tumor Targeting.
Two derivatives of hyaluronic acid (HA) have been synthesized by atom transfer radical polymerization (ATRP), starting from an ethylenediamino HA derivative (HA-EDA) and by using diethylaminoethyl methacrylate (DEAEMA) as a monomer for polymerization. Both samples, indicated as HA-EDA-pDEAEMA a and b, are able to condense siRNA, as determined by gel retardation assay and resulting complexes show a size and a zeta potential value dependent on polymerization number, as determined by dynamic light scattering measurements. In vitro studies performed on HCT 116 cell line, that over express CD44 receptor, demonstrate a receptor mediated uptake of complexes, regardless of their surface charge. New…
Inulin for Cancer Therapy: Present and Perspectives
Inulin is an extremely adaptable polysaccharides consisting of glucopyranose end-capped (β-1,2) fructose repeating units and, as it is, can be classified as an inherently multifunctional polymeric scaffold. It may be further functionalized employing mild conditions to give rise desired biological and physicochemical properties exploitable for targeted anticancer applications (e.g., active targeting toward specific cytotypes, self-assembling behavior, selective cytoxicity and hyperthermia features). In this review, the main chemical features and the inulin derivatives applications in the field of targeted anticancer therapy is reported and discussed
MULTIFUNCTIONAL HYALURONIC ACID DERIVATIVE FOR EASY SCAFFOLD PROCESSING AND BIOLOGICAL FUNCTIONALIZATION
POLISACCARIDI FUNZIONALIZZATI CON PORZIONI ALCHILICHE: SINTESI, CARATTERIZZAZIONE REOLOGICA ED APPLICAZIONI BIOMEDICHE
Fibrillar polymeric patches suitable for wound healing
Multilayered scaffolds can be designed to improve soft material applicability for regenerative medicine purposes. Excellent results can be obtained by combining the main characteristics of the biopolymers and giving a specific function to each layer. A microfluidic-assisted electrospinning process is an elegant arrangement between two fiber fabrication techniques that defines finely every portion of an electrospun scaffold. Unlike classic electrospinning technique, multiple flows can be carefully monitored and separated in real time using microfluidic sensors. This method allows users to establish anisotropic properties to scaffolds, in other words it is possible to modulate gradients by ch…
HYALURONIC ACID/-CYCLODEXTRINS HYDROGEL FOR CORNEAL RELEASE OF EPITHELIAL CELLS AND DEXAMETHASONE
INJECTABLE HYDROGEL BASED ON A NOVEL AMPHIPHILIC HYALURONIC ACID DERIVATIVE FOR CONTROLLED DRUG RELEASE
LIPID NANOPARTICLES AS CARRIERS FOR TARGETED RELEASE OF RILUZOLE INTO THE CENTRAL NERVOUS SYSTEM
Electrospun Plasma-assisted Functionalized Ocular Inserts for Triamcinolone Acetonide Delivery
Topically administered ocular Drug Delivery Systems are the most desirable formulations for the treatment of ocular chronic diseases, being the most simple, convenient and patient-friendly ocular route of administration, despite it requires prolonged and continuous treatment over time. At this regard, ocular insert represent an innovative and effective strategy. Ocular inserts are solid/semisolid sterile preparations characterized by dimensions and shape specifically designed for topical ocular administration. They are able to rapidly adhere on the ocular surface and remain in situ for prolonged period while assuring a controlled drug release and potentially increasing drug transcorneal abs…
DRUG RELEASE FROM INULIN DERIVATIVE HYDROGEL: EFFECT OF DRUG LOADING AND pH
NUOVI SISTEMI POLIMERICI A STRUTTURA POLISACCARIDICA/POLIAMMINOACIDICA IMPIEGATI PER IL RILASCIO COLON-SPECIFICO DI ANTICORPI MONOCLONALI
Smart inulin-based polycationic nanodevices for siRNA delivery
The advances of short interfering RNA (siRNA) mediated therapy provide a powerful option for the treatment of many diseases by silencing the expression of targeted genes including cancer development and progression. Inulin is a very simple and biocompatible polysaccharide proposed by our groups to produce interesting delivery systems for Nucleic Acid Based Drugs (NABDs), such as siRNA, either as polycations able to give polyplexes and polymeric coatings for nanosystems having a metallic core. In this research field, different functionalizing groups were linked to the inulin backbone with specific aims including oligoamine such as Ethylendiammine (EDA), Diethylediamine (DETA), Spermine, (SPM…
COMPLEXES OF IRON WITH INULIN DERIVATIVES: A NEW STRATEGY IN THE TREATMENT OF IRON DEFICIENCY ANAEMIA
SPRAY DRIED MICROPARTICLES OF A NEW HYALURONIC ACID DERIVATIVE FOR POTENTIAL BIO-INSTRUCTION OF STAMINAL CELLS
HYALURONIC ACID-BETA CYCLODEXTRINS HYDOGEL FOR THE TREATMENT OF SEVERE CORNEAL DISEASES
PRODUCTION VIA MICROFLUIDICS OF FIBRILLAR AND NANOMETRIC BIOMATERIALS FOR REGENERATIVE MEDICINE AND DRUG DELIVERY POURPOSES
Microfluidic technique offers mild operating conditions suitable for fabrication of fibers and cell encapsulation and can be applied for controlled production of nanometric biomaterials1 . Ionotropic polysaccharides are routinely employed for microfluidics. To be processed, nonionotropic polysaccharides, such as Hyaluronic Acid (HA), should be chemically crosslinked during fabrication2 . In order to avoid chemical crosslinking, we designed an ionic strenght sensible HA derivative suitable for microfluidics2 . Here we describe the use of such HA derivative for fabrication of fibrillar and nanometric biomaterials for regenerative medicine and drug delivery applications. For muscle tissue rege…
Biocompatibility and biodegradability of electrospun phea-pla scaffolds: Our preliminary experience in a murine animal model
We obtained a nano-fibrillar scaffold starting from a polymeric solution which, through electrospinning, gave a biodegradable material with optimal mechanical features and the capacity to allow cell adhesion. In this paper we report the in-vivo application on a murine animal model of two electrospun biodegradable materials, specifically designed to create tubular structures. In one case PHEA-PLA was co-spun with silk fibroin (Fibro-PHEAPLA) by a parallel electrospinning process to obtain a scaffold with two different polymeric fibers. In the other case, PHEA-PLA was mixed with polycaprolactone (PCLPHEA-PLA) to obtain a hybrid fibers scaffold. The in-vitro assay showed fibroblast colonizatio…
Graphene nanosystems as supports in siRNA Delivery
The nature of graphene-based nanosystems, as well as the possibility to synthesize them at low cost, have made them in the last few years, an interesting proposition in tumour therapy as a drug delivery system. Here, a reduced form of graphene oxide (RGO) has been synthesized and conjugated with a specific antibody for active targeting against tumour cells (RGOY). Furthermore, its bi-dimensional nature permits also p- p stacking interactions with planar molecules like siRNA (RGOY-siRNA). All these nano-complexes were characterized by DLS and DSC analysis, TEM and Raman spectroscopy and their biocompatibility was demonstrated by viability assay on cell line ECV 304. The system is able to be …
NOVEL AMPHIPHILIC COPOLYMERS BASED ON POLYASPARTAMIDE GRAFT WITH SQUALENE RESIDUES TO OBTAIN MICELLES AS COLLOIDAL DRUG CARRIERS.
New polysaccharide-polyaminoacid hydrogels: a suitable strategy for colon-specific drug delivery
The aim of this study was to prepare and characterize novel hydrogels with polysaccharide–polyaminoacid structure, able to undergo an enzymatic hydrolysis in the colon and potentially useful for treating inflammatory bowel diseases (IBD). Starting materials were methacrylated dextran (DEX-MA) and methacrylated α,β-poly(N-2-hydroxyethyl)-DL-aspartamide (PHM). These polymers were photocrosslinked by exposure of their aqueous solutions at 313 nm without photoinitiators. Different samples, shaped as microparticles, were obtained as a function of polymer concentration and irradiation time. FT-IR analysis confirmed the occurrence of a co-crosslinking between DEX-MA and PHM in all experimental con…