0000000000543881
AUTHOR
Alessia Grasso
Hsa-miR-30d, secreted by the human endometrium, is taken up by the pre-implantation embryo and might modify its transcriptome.
During embryo implantation, the blastocyst interacts with and regulates the endometrium, and endometrial fluid secreted by the endometrial epithelium nurtures the embryo. Here, we propose that maternal microRNAs (miRNAs) might act as transcriptomic modifier of the pre-implantation embryo. Microarray profiling revealed that six of 27 specific, maternal miRNAs were differentially expressed in the human endometrial epithelium during the window of implantation – a brief phase of endometrial receptivity to the blastocyst – and were released into the endometrial fluid. Further investigation revealed that hsa-miR-30d, the expression levels of which were most significantly upregulated, was secreted…
Analysis of miRNAs in endometrial fluid as a tool for non-invasive diagnosis of endometrial receptivity
La implantación embrionaria es el proceso por el que el embrión humano en fase de blastocisto se adhiere al útero materno para comenzar la gestación. El endometrio, la mucosa uterina solo puede aceptar al embrión en un momento determinado del ciclo menstrual que puede variar entre 12 horas y 2 días, identificado como "ventana de implantación". Desde siempre se ha buscado la forma de identificar de forma fiable dicha ventana utilizando técnicas histológicas, como los criterios de Noyes, a través de la observación morfológica vía ecográfica, o estudiando los cambios en los perfiles transcriptómicos, proteómicos o metabolómicos. No obstante, en reproducción asistida, se está abriendo el camino…