0000000000547832

AUTHOR

Roger J. Price

showing 2 related works from this author

Metabolism of coumarin by precision-cut calf liver slices and calf liver microsomes.

1995

1. The metabolism of 50 microM [3-14C]coumarin has been studied in precision-cut-calf liver slices. 2. The metabolism of 50 microM coumarin to 7-hydroxycoumarin has also been examined in calf, rat, Cynomolgus monkey and human liver microsomal preparations. 3. In precision-cut calf liver slices, [3-14C]coumarin was metabolized to various polar products and to metabolite(s) that bound covalently to calf liver slice proteins. The polar products included 7-hydroxycoumarin (which was extensively conjugated with D-glucuronic acid and/or sulphate), metabolites of the 3-hydroxylation pathway (mainly o-hydroxyphenylethanol and o-hydroxyphenylacetic acid), and unknown metabolites. 4. Coumarin 7-hydro…

MaleHealth Toxicology and MutagenesisMetaboliteIn Vitro TechniquesToxicologyBiochemistryRats Sprague-Dawleychemistry.chemical_compoundSpecies SpecificityCoumarinsmedicineAnimalsHumansPharmacologybiologyMethoxsalenReproducibility of ResultsGeneral MedicineMetabolismbiology.organism_classificationCoumarinEnzyme assayRatsMacaca fascicularischemistryMicrosomaBiochemistryLiverMicrosomebiology.proteinMicrosomes LiverCattleDrug metabolismmedicine.drugXenobiotica; the fate of foreign compounds in biological systems
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Induction of cytochrome P450 isoenzymes in cultured precision-cut rat and human liver slices

1996

1. The effect of some xenobiotics on levels of selected cytochrome P450 (CYP) isoenzymes determined by Western immunoblotting and associated enzyme activities has been studied in 72-h cultured rat and human precision-cut liver slices. 2. In cultured rat liver slices, 0.5 mM sodium phenobarbitone (PB), 25 microM beta-naphthoflavone (BNF), and 20 micrograms/ml Aroclor 1254 (ARO) induced mixed-function oxidase enzyme activities. Western immunoblotting of liver slice microsomes was performed with antibodies to rat CYP1A2, 2B1/2 and 3A. Compared with 72-h control (dimethyl sulphoxide only treated) rat liver slice microsomes, PB induced CYP2B1/2 and 3A, BNF induced CYP1A2, and ARO induced CYP1A2,…

MaleAroclorsmedicine.medical_specialtyHealth Toxicology and MutagenesisToxicologyMicrobodiesBiochemistryIsozymeRats Sprague-DawleyClofibric AcidCytochrome P-450 Enzyme Systembeta-NaphthoflavoneCulture TechniquesInternal medicinemedicineAnimalsHumansEnzyme inducerBenzoflavonesPharmacologychemistry.chemical_classificationOxidase testbiologyFibric AcidsCytochrome P450General MedicineChlorodiphenyl (54% Chlorine)In vitroRatsIsoenzymesPyrimidinesEndocrinologyEnzymeLiverchemistryEnzyme InductionPhenobarbitalClofenapatebiology.proteinMicrosomeCiprofibratemedicine.drugXenobiotica
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