0000000000548960

AUTHOR

Arianna Ragona

showing 2 related works from this author

Evolutionary Analysis of Grapevine Virus A: Insights into the Dispersion in Sicily (Italy)

2022

Grapevine virus A (GVA) is a phloem-restricted virus (genus Vitivirus, family Betaflexiviridae) that cause crop losses of 5–22% in grapevine cultivars, transmitted by different species of pseudococcid mealybugs, the mealybug Heliococcus bohemicus, and by the scale insect Neopulvinaria innumerabilis. In this work, we studied the genetic structure and molecular variability of GVA, ascertaining its presence and spread in different commercial vineyards of four Sicilian provinces (Italy). In total, 11 autochthonous grapevine cultivars in 20 commercial Sicilian vineyards were investigated, for a total of 617 grapevine samples. Preliminary screening by serological (DAS-ELISA) analysis for GV…

grapevine disease; GVA; <i>Vitivirus</i>; <i>Betaflexiviridae</i>; DAS-ELISA; RT-PCR; phylogenetic analysisGrapevine disease GVA Vitivirus Betaflexiviridae DAS-ELISA RT-PCR Phylogenetic analysisPlant ScienceAgronomy and Crop ScienceFood ScienceAgriculture
researchProduct

Epidemiological Survey of Grapevine Leafroll-Associated Virus 1 and 3 in Sicily (Italy): Genetic Structure and Molecular Variability

2022

Background: the most widely distributed and virulent Grapevine leafroll-associated viruses (GLRaV) that affect grapevine are GLRaV-1 and GLRaV-3, transmitted semi-persistently by different mealybugs and soft scales, mainly causing downward rolling of the leaf margins and interveinal reddening. Methods: the main objectives of this study were to investigate the genetic structure and molecular diversity of GLRaV-1 and GLRaV-3 in 617 samples from 11 autochthonous Sicilian grapevine cultivars, ascertaining their presence and spread. The detection was implemented by serological and molecular analyses and subsequently phylogenetic analyses on selected Sicilian isolates were conducted. Results: in …

grapevine disease; GLRaV-1; GLRaV-3; <i>Closteroviridae</i>; <i>Ampelovirus</i>; DAS-ELISA; RT-PCR; phylogenetic analysesPlant ScienceAgronomy and Crop Sciencegrapevine disease GLRaV-1 GLRaV-3 Closteroviridae Ampelovirus DAS-ELISA RT-PCR phylogenetic analysesFood ScienceAgriculture; Volume 12; Issue 5; Pages: 647
researchProduct