0000000000559129

AUTHOR

Hadi Lashini

0000-0003-0373-6315

showing 1 related works from this author

Use of TaqMan® real-time PCR for rapid detection of Salmonella enterica serovar Typhi

2014

We evaluated the performances of a newly designed real-time polymerase chain reaction (PCR) assay using TaqMan® probes to detectSalmonellaTyphi. TaqMan® real-time PCR assays were performed by designed primers and probe based on thestaGgene for detectingS.Typhi. The specificity of the assay was evaluated on 15Salmonellaserovars. The analytical specificity was evaluated on 20 non-Salmonellamicroorganisms. The analytical sensitivity was assessed using decreasing DNA quantities ofS.Typhi ATCC 19430. Finally the detection capability of the TaqMan® real-time PCR assay on isolates recovered from patients withSalmonellainfections was compared to the conventional PCR assay. OnlyS.Typhi strain had po…

SerotypeSalmonellaSettore MED/07 - Microbiologia E Microbiologia ClinicaBiologySalmonella typhimedicine.disease_causeReal-Time Polymerase Chain ReactionSettore MED/42 - Igiene Generale E ApplicataRapid detectionSensitivity and Specificitylaw.inventionlawTaqManmedicineHumansTyphoid FeverPolymerase chain reactionreal time typhoid fever diagnosisDNA PrimersGeneral Immunology and MicrobiologyGeneral MedicineSalmonella typhiVirologyMolecular biologyBacterial Typing TechniquesReal-time polymerase chain reactionSalmonella enterica serovar TyphiGenes Bacterial
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