0000000000560159

AUTHOR

Toufic Elbeaino

showing 3 related works from this author

Viruses infecting different Mediterranean genotypes of Ficus carica and their distribution in different plant organs.

2017

Seven Mediterranean Ficus carica genotypes, i.e. cv. Palazzo, Severoni precoce, Bianca, Pilusedda, Dottato bianco, Bifara and Zidi, were screened for the presence of seven fig-infecting viruses associated with fig mosaic disease (FMD) in order to explore their distribution in different plant organs (leaf, bud and syconium) that will be utilized as a plant source material in different sanitation techniques. RT-PCR assays conducted on reverse-transcribed TNA extracted from leaves, apical buds and syconia (1.5-2cm) of each genotype for the presence of Fig leaf mottle-associated virus 1 (FLMaV-1), Fig mild mottling-associated virus (FMMaV), Fig mosaic virus (FMV), Fig latent virus 1 (FLV-1), Fi…

Mediterranean climatebiologybusiness.industryDistribution (economics)FicusSettore AGR/12 - Patologia VegetaleHorticultureFig mosaic RT-PCR virus distributionbiology.organism_classificationSettore AGR/03 - Arboricoltura Generale E Coltivazioni ArboreeHorticultureGenotypeBotanyCaricabusiness
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Fig virus-free production and survival rate improvement using meristem tip culture techinique associated with the encapsulation technology

2018

Three Mediterranean F. carica genotypes, i.e. cultivars Palazzo, Bifera nera and Catalanisca, initially infected by Fig leaf mottle-associated virus 1 (FLMaV-1), Fig leaf mottle-associated virus 2 (FLMaV-2), Fig mild mottling-associated virus (FMMaV), Fig mosaic virus (FMV), Fig latent virus 1 (FLV-1), Fig Badnavirus 1 (FBV-1) and Fig fleck-associated virus (FFkaV), were subjected to the sanitation technique via Meristem Tip (0.3-0.5 mm in size) Culture (MTC), also associated with the encapsulation technique (MTC-SS), in order to produce virus-free plant material. Encapsulation was tested to overcome the very low survival and regeneration rates, due to the small propagule size. Encouraging …

0106 biological sciencesChemistryfig mosaic disease synthetic seed sanitation RT-PCR.Settore AGR/12 - Patologia Vegetale04 agricultural and veterinary sciencesHorticultureMeristem01 natural sciencesEncapsulation (networking)Cell biologySettore AGR/03 - Arboricoltura Generale E Coltivazioni Arboree040103 agronomy & agriculture0401 agriculture forestry and fisheriesVirus freeSurvival rate010606 plant biology & botany
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The sequencing of the complete genome of a Tomato black ring virus (TBRV) and of the RNA2 of three Grapevine chrome mosaic virus (GCMV) isolates from…

2014

The complete genome of a Tomato black ring virus isolate (TBRV-Mirs) (RNA1, 7,366 nt and RNA2, 4,640 nt) and the RNA2 sequences (4,437; 4,445; and 4,442 nts) of three Grapevine chrome mosaic virus isolates (GCMV-H6, -H15, and -H27) were determined. All RNAs contained a single open reading frame encoding polyproteins of 254 kDa (p1) and 149 kDa (p2) for TBRV-Mirs RNA1 and RNA2, respectively, and 146 kDa for GCMV RNA2. p1 of TBRV-Mirs showed the highest identity with TBRV-MJ (94 %), Beet ringspot virus (BRSV, 82 %), and Grapevine Anatolian ringspot virus (GARSV, 66 %), while p2 showed the highest identity with TBRV isolates MJ (89 %) and ED (85 %), followed by BRSV (65 %), GCMV (58 %), and GA…

Sequence analysisMolecular Sequence DataNepovirusGenome ViralBiologyDNA sequencingGrapevine chrome mosaic viruslaw.inventionOpen Reading FramesSolanum lycopersicumlawVirologyPlant virusGeneticsCluster AnalysisVitisGrapevine chrome mosaic virusMovement proteinLycopersicon esculentumMolecular BiologyPhylogenyRecombination analysisPolyproteinsRecombination GeneticSequence Homology Amino AcidSequence analysisTomato black ring virusGeneral MedicineSequence Analysis DNATomato black ring virusbiology.organism_classificationVirologyMolecular WeightGenBankRecombinant DNARNA ViralGrapevine
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