0000000000564190

AUTHOR

Hubert Kalbacher

showing 2 related works from this author

DRB1*0401-restricted human T cell clone specific for the major proinsulin73-90 epitope expresses a down-regulatory T helper 2 phenotype.

2006

Recently, we have identified proinsulin (P-Ins) 73-90 as an immunodominant T cell epitope of HLA-DRB1*0401 (DR4) subjects with β-islet cell autoimmunity and of HLA-DR4/CD4 double-transgenic mice immunized with human P-Ins. We have compared the fine specificities of one human CD4 T cell clone and two mouse T cell hybridoma clones recognizing this epitope, and, although these three clones all recognized the same core region (LALEGSLQK), there were major differences in how they interacted with the peptide (p)/HLA complex, reflecting the fact that human P-Ins is a foreign antigen in the mouse and an autoantigen in the type 1 diabetes patient. The human T cell clone was forkhead transcription f…

Regulatory T cellT cellT-LymphocytesMolecular Sequence DataClone (cell biology)Mice TransgenicHuman leukocyte antigenBiologyEpitopeEpitopesMiceAntigenT-Lymphocyte SubsetsmedicineCytotoxic T cellAnimalsHumansAmino Acid SequenceAmino AcidsMultidisciplinaryFOXP3Forkhead Transcription FactorsHLA-DR AntigensBiological SciencesMolecular biologyPeptide Fragmentsmedicine.anatomical_structurePhenotypeHLA-DRB1 ChainsProinsulinProceedings of the National Academy of Sciences of the United States of America
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Presentation of insulin and insulin A chain peptides to mouse T cells: involvement of cysteine residues.

1991

The requirements for insulin presentation and recognition by A alpha b A beta b- and A alpha b A beta k-restricted mouse T cells were studied using a variety of derivatives of the insulin A chain. It was found that A chain peptides with irreversibly blocked Cys residues are non-stimulatory for the T cells. This suggests that at least one of the Cys residues is essential for recognition. On the other hand, all A chain peptides containing Cys residues modified in a way reversible by reaction with thiols are stimulatory yet differ in antigenic potency. All these A chain derivatives including a 14 amino acid fragment require uptake by antigen presenting cells (APC) for efficient presentation. D…

Swinemedicine.medical_treatmentT-LymphocytesImmunologyAntigen presentationReceptors Antigen T-CellAntigen-Presenting CellsPeptideMice Inbred StrainsIn Vitro TechniquesCell LineEpitopesMiceAntigenmedicineAnimalsInsulinCysteineAntigen-presenting cellMolecular Biologychemistry.chemical_classificationChemistryInsulinT-cell receptorHistocompatibility Antigens Class IIChloroquineAmino acidBiochemistryCattleInterleukin-3PeptidesCysteineMolecular immunology
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