0000000000583316

AUTHOR

C. Sprössler

showing 3 related works from this author

Model network architectures in vitro on extracellular recording systems using microcontact printing

2001

A PDMS stamp is used to transfer a synthetic peptide in a given pattern to any suitable surface. Using this method two-dimensional neuronal model networks could be formed on glass substrates as well as on electronic devices and adjusted to the given microelectronic structure. The present work focuses on the mechanism of neurite guidance under simplified in vitro conditions, using in vitro guidance cues and outline the incorporation of these interfacial methods into microelectronic sensor devices.

Materials sciencebusiness.industryMechanical EngineeringMetals and AlloysPDMS stampNanotechnologyCondensed Matter PhysicsElectronic Optical and Magnetic MaterialsMechanics of MaterialsMicrocontact printingMaterials ChemistryMicroelectronicsModel networkElectronicsbusinessBiosensor
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Ordered networks of rat hippocampal neurons attached to silicon oxide surfaces.

2001

The control of neuronal cell position and outgrowth is of fundamental interest in the development of applications ranging from cellular biosensors to tissue engineering. We have produced rectangular networks of functional rat hippocampal neurons on silicon oxide surfaces. Attachment and network formation of neurons was guided by a geometrical grid pattern of the adhesion peptide PA22-2 which matches in sequence a part of the A-chain of laminin. PA22-2 was applied by contact printing onto the functionalised silicon oxide surface and was immobilised by hetero-bifunctional cross-linking with sulfo-GMBS. Geometric pattern matching was achieved by microcontact printing using a polydimethylsiloxa…

Cell Culture TechniquesNanotechnologyBiosensing TechniquesHippocampusMembrane Potentialschemistry.chemical_compoundFetusmedicineBiological neural networkCell AdhesionAnimalsSilicon oxideCells CulturedCell SizeMembrane potentialNeuronsPolydimethylsiloxaneChemistryGeneral NeuroscienceSilicon CompoundsPDMS stampOxidesAdhesionRatsElectrophysiologymedicine.anatomical_structureMicrocontact printingBiophysicsNeuronNerve NetPeptidesJournal of neuroscience methods
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Extended gate electrode arrays for extracellular signal recordings

2000

Abstract We have fabricated arrays of planar gold electrodes arranged in a matrix of 8×8 with active areas ranging from 6 to 30 μm in diameter. An electronic amplification circuitry based on commercial junction field-effect transistors was used where the gold sensor fields act as extended gate electrodes (EGE) of the transistors, which leads to a new approach for long-term extracellular recording systems in vitro. The high input resistance of the amplification circuitry allows the use of small planar bare gold electrodes without further modification which therefore extends the frequency range of the measuring set-up down to the DC-level. The performance of our recording system has been test…

Materials sciencebusiness.industryTransistorMetals and AlloysAnalytical chemistryRangingCondensed Matter PhysicsCapacitanceSurfaces Coatings and FilmsElectronic Optical and Magnetic Materialslaw.inventionMatrix (chemical analysis)PlanarExtracellular signalSensor arraylawElectrodeMaterials ChemistryOptoelectronicsElectrical and Electronic EngineeringbusinessInstrumentationSensors and Actuators B: Chemical
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