0000000000584382
AUTHOR
Claudia Mazzarella
Myc Promoter-Binding Protein-1 (MBP-1) Is a Novel Potential Prognostic Marker in Invasive Ductal Breast Carcinoma
BackgroundAlpha-enolase is a glycolytic enzyme that catalyses the formation of phosphoenolpyruvate in the cell cytoplasm. α-Enolase and the predominantly nuclear Myc promoter-binding protein-1 (MBP-1) originate from a single gene through the alternative use of translational starting sites. MBP-1 binds to the P2 c-myc promoter and competes with TATA-box binding protein (TBP) to suppress gene transcription. Although several studies have shown an antiproliferative effect of MBP-1 overexpression on several human cancer cells, to date detailed observations of α-enolase and MBP-1 relative expression in primary tumors versus normal tissues and their correlation with clinicopathological features ha…
Negative transcriptional control of ERBB2 gene by MBP-1 and HDAC1: diagnostic implications in breast cancer
Abstract Background The human ERBB2 gene is frequently amplified in breast tumors, and its high expression is associated with poor prognosis. We previously reported a significant inverse correlation between Myc promoter-binding protein-1 (MBP-1) and ERBB2 expression in primary breast invasive ductal carcinoma (IDC). MBP-1 is a transcriptional repressor of the c-MYC gene that acts by binding to the P2 promoter; only one other direct target of MBP-1, the COX2 gene, has been identified so far. Methods To gain new insights into the functional relationship linking MBP-1 and ERBB2 in breast cancer, we have investigated the effects of MBP-1 expression on endogenous ERBB2 transcript and protein lev…
Conoscenze , attitudini e comportamenti del personale addetto alla ristorazione in un ospedale siciliano”, XIV Congresso Interregionale Calabro-Siculo .Isola di Capo Rizzuto (CZ), 28-30 maggio 2004
Ciona intestinalis interleukin 17-like genes expression is upregulated by LPS challenge
In humans, IL-17 is a proinflammatory cytokine that plays a key role in the clearance of extracellular bacteria promoting cell infiltration and production of several cytokines and chemokines. Here, we report on three Ciona intestinalis IL-17 homologues (CiIL17-1, CiIL17-2, CiIL17-3). The gene organisation, phylogenetic tree and modelling supported the close relationship with the mammalian IL-17A and IL-17F suggesting that the C. intestinalis IL-17 genes share a common ancestor in the chordate lineages. Real time PCR analysis showed a prompt expression induced by LPS inoculation suggesting that they are involved in the first phase of inflammatory response. In situ hybridization assays disclo…
Applicazione della PFGE nella sorveglianza da gram negativi multiresistenti in UTIN”, I Congresso Nazionale SIMPIOS; Bergamo, 20-22 maggio 2004
Nuclear Myc Promoter-Binding Protein-1 (MBP-1) Expression Is a Prognostic Factor in Invasive Ductal Breast Carcinoma
Colonizzazione da gram negativi multiresistenti produttori di ESBL in una UTIN”, I Congresso Nazionale SIMPIOS; Bergamo, 20-22 maggio 2004;
THAP11 and HP1BP3 proteins are component of the HB-2.8 enhancer-blocker located in the human c-MYC chromatin domain.
Colonizzazione da Gram negativi multiresistenti produttori di ESBL in una UTIN.
CMYC PROMOTER BINDINGPROTEIN 1 (MBP1) AS A NOVEL MARKER TO IDENTIFY NEW SUBTYPES OF ERBB2 NEGATIVE BREAST TUMORS
AMPs and biotechnology application for new generation of medical devices
Valutazione della qualità igienica di pasti pronti per il consumo, forniti da servizi di catering a Palermo”; 41° Congresso Nazionale SITI, Genova, 20-23 ottobre 2004;
Gestione di un evento epidemico in un reparto ad alto rischio
Interleukin 17 genes as mediators of inflammatory responses in Ciona intestinalis.
Inflammation is a complex reaction of host defence mechanisms aiming at neutralization of an insult and restoring normal tissue structure and function. In human IL-17 is T-cell derived cytokine plays a key role in the clearance of extracellular bacteria promoting cell infiltration and production of several cytokines and chemokines. Here, we report on three Ciona intestinalis IL-17 homologues (CiIL17-1, CiIL17-2, CiIL17-3). The gene organization, phylogenetic tree and modeling supported the close relationship with the mammalian IL-17A and IL-17F suggesting that the C. intestinalis IL-17 genes share a common ancestor in the chordate lineages. Real time PCR analysis showed a prompt expression …
Nuclear myc promoter-binding protein-1 (MBP-1) expression is a prognostic factor in invasive ductal breast carcinoma
Insulators are DNA elements that block the extension of a condensed chromatin domain into a transcriptionally active region or prevent the interaction of a distal enhancer with a promoter when placed between the two. Human c-Myc and Pvt-1 genes map close each other and are separated by an intergenic region rich of DNAseI hypersensitivity sites. Starting from the observation that PVT1 expression is restricted to a low number of normal tissues compared to the wide distribution of c-Myc mRNA we focus our studies on the function and structure of the regions surrounding the DHs present between the two genes. Stable and transient transfection, indicate that one of the regions (HB-2.8) has enhance…