0000000000588133

AUTHOR

Sibylle Kaiser

showing 3 related works from this author

Cloning and Targeted Deletion of the Mouse Fetuin Gene

1998

We proposed that the alpha2-Heremans Schmid glycoprotein/fetuin family of serum proteins inhibits unwanted mineralization. To test this hypothesis in animals, we cloned the mouse fetuin gene and generated mice lacking fetuin. The gene consists of seven exons and six introns. The cystatin-like domains D1 and D2 of mouse fetuin are encoded by three exons each, whereas a single terminal exon encodes the carboxyl-terminal domain D3. The promoter structure is well conserved between rat and mouse fetuin genes within the regions shown to bind transcription factors in the rat system. Expression studies demonstrated that mice homozygous for the gene deletion lacked fetuin protein and that mice heter…

alpha-2-HS-GlycoproteinMolecular Sequence DataBiologyBiochemistryMiceEctopic calcificationExonCalcification PhysiologicApatitesmedicineAnimalsCloning MolecularPromoter Regions GeneticMolecular BiologyGeneMice Knockoutchemistry.chemical_classificationBase SequenceIntronBlood ProteinsSequence Analysis DNACell Biologymedicine.diseaseNull alleleMolecular biologyFetuinRatschemistryFemalealpha-FetoproteinsGlycoproteinalpha-2-HS-glycoproteinGene DeletionJournal of Biological Chemistry
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Genetic and molecular analysis of six tumor suppressor genes in Drosophila melanogaster

1990

Six Drosophila melanogaster tumor suppressor genes causing malignant or benign tumors in specific cell types are described. The wild-type alleles of these genes are instrumental in the differentiation of particular cell types. In the homozygous state, recessive mutations in the genes interrupt the differentiation of the cells and thus cause their uncontrolled, autonomous, lethal proliferation. The tumors show all major characteristics of malignant and benign neoplastic growth. Genomic sequences of four of the genes have been identified and are currently being characterized. ImagesFIGURE 1.FIGURE 2.FIGURE 2.

GeneticsCell typebiologyHealth Toxicology and MutagenesisRestriction MappingPublic Health Environmental and Occupational HealthNeoplastic growthNeoplasms Experimentalbiology.organism_classificationMolecular analysislaw.inventionMolecular and Cellular Aspects of Transformation and DifferentiationRestriction mapDrosophila melanogasterlawSuppressorAnimalsGenes LethalGenes Tumor SuppressorDrosophila melanogasterAlleleGeneEnvironmental Health Perspectives
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Induction of bone morphogenetic protein-6 in skin wounds. Delayed reepitheliazation and scar formation in BMP-6 overexpressing transgenic mice.

1998

Growth factors of the transforming growth factor-beta superfamily are involved in cutaneous wound healing. In this study we analyze the expression of the bone morphogenetic protein-6 (BMP-6) gene, a transforming growth factor-beta related gene, in skin wounds. In normal mouse skin high levels of BMP-6 mRNA and protein are expressed by postmitotic keratinocytes of stratified epidermis until day 6 after birth. BMP-6 expression is strongly reduced in adult epidermis with diminished mitotic activity. After skin injury we found large induction of BMP-6-specific RNA and protein in keratinocytes at the wound edge and keratinocytes of the newly formed epithelium as well as in fibroblast shaped cell…

AdultPathologymedicine.medical_specialtyBone Morphogenetic Protein 6Gene ExpressionMice TransgenicDermatologyBiologyBiochemistryMiceTransforming Growth Factor betaGene expressionmedicineAnimalsHumansFibroblastMolecular BiologySkinMessenger RNAWound Healingintegumentary systemEpidermis (botany)RNACell DifferentiationCell BiologyCell biologyUp-RegulationBone morphogenetic protein 6medicine.anatomical_structureBone Morphogenetic ProteinsRNAWounds and InjuriesWound healingCell DivisionTransforming growth factorThe Journal of investigative dermatology
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