0000000000588888
AUTHOR
Francesca Timoneri
showing 20 related works from this author
CD40 activation in human pancreatic islets and ductal cells.
2008
Aims/hypothesis: CD40 expression on non-haematopoietic cells is linked to inflammation. We previously reported that CD40 is expressed on isolated human and non-human primate islets and its activation results in secretion of IL-8, macrophage inflammatory protein 1-beta (MIP-1β) and monocyte chemoattractant protein-1 (MCP-1) through nuclear factor-κB and extracellularly regulated kinases 1/2 pathways. The objective of this study was to identify the pattern of gene expression, and to study viability and functionality affected by CD40-CD40 ligand (CD40L) interaction in human islets. Furthermore, we have studied the CD40-mediated cytokine/chemokine profile in pancreatic ductal cells, as they are…
Wharton’s Jelly Mesenchymal Stromal Cells from Human Umbilical Cord: a Close-up on Immunomodulatory Molecules Featured In Situ and In Vitro
2019
Therapeutic options for end-stage organ failure are often limited to whole organ transplantation. The tolerance or rejection of the transplanted organ is driven by both early non-specific innate and specific adaptive responses. The use of mesenchymal stromal cells (MSCs) is considered a promising tool in regenerative medicine. Human umbilical cord (HUC) is an easily available source of MSCs, without relevant ethical issues. Moreover, Wharton's jelly-derived MSCs (WJ-MSCs), showed consistent immunomodulatory features that may be useful to promote immune tolerance in the host after transplantation. Few data are available on the phenotype of WJ-MSCs in situ. We investigated the expression of i…
Prolactin supplementation to culture medium improves beta-cell survival
2010
OBJECTIVES.: Recent studies demonstrated that prolactin (PRL) has beneficial effects on β cells for islet transplantation. We examined the effect of human recombinant PRL (rhPRL) supplementation to the culture media to determine its potential use in the context of clinical islet transplantation. MATERIALS AND METHODS.: Each human islet isolated from 14 deceased multiorgan donors was cultured in Miami modified media-1 supplemented with or without rhPRL (500 μg/L) for 48 hr. β-Cell survival and proliferation (BrdU and Ki-67) were determined by laser scanning cytometry. The cytoprotective effects of rhPRL against noxious stimuli were assessed by flow cytometry (tetramethylrhodamine ethyl ester…
Isolation and phenotypical characterization of mesenchymal stem cells from the Wharton's jelly of pre-term human umbilical cord.
2014
ISOLATION AND PHENOTYPICAL CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM PRE-TERM HUMAN UMBILICAL CORD MATRIX
2012
Hepatocyte-like cells differentiated from Wharton's jelly mesenchymal stem cells: functional characterization and expression of immunomodulatory mole…
2014
Characterization of the in vitro immunomodulatory properties of mesenchymal stem cells isolated from Wharton's jelly: new actors at play
2014
Wharton's jelly mesencymal stem cells differentiated into hepatocyte-like cells show expression of immunomodulatory molecules
2014
Isolation and phenotypical characterization of mesenchymal stem cells from the Wharton's jelly of pre-term human umbilical cord
2014
Wharton’s jelly mesenchymal stem cells differrentiation towards hepatocyte-like cells: in vitro evidences
2012
Shifting back the fetomaternal interface: WHarton's jelly mesenchymal stem cells immunomodulatory molecules and their journey from umbilical cord to …
2014
Wharton's jelly immunomodulatory properties and unique markers expression: new actors at play
2014
Characterization of the immunomodulatory properties of mesenchymal stem cells isolated from Wharton's jelly
2012
Wharton's jelly mesenchymal stem cells differentiation towards hepatocyte-like cells: functional characterization and expression of immunomodulatory …
2012
Wharton's jelly mesenchymal stem cells differentiation towards hepatocyte-like cells: functional characterization and expression of immunomodulatory …
2014
Wharton’s Jelly Mesenchymal Stem Cells from Preterm Umbilical Cords: Isolation, Characterization and Potential Use as Extrahepatic Progenitors
2014
Wharton’s jelly mesenchymal stem cells differentiation into hepatocyte-like cells: functional characterization and expression of immunomodulatory mol…
2015
Mesenchymal stem cells derived from Wharton’s jelly (WJ-MSCs) recently emerged as promising tools for cellular therapy due to their ability to differentiate into diverse cell types and their immunomodulatory features. Little is known on the expression of immunomodulatory molecules in mature cells differentiated from WJ-MSCs, therefore we aimed to characterize the extent of maintenance of the naive traits of these cells also in a highly specialized differentiated counterpart. WJ-MSCs were differentiated into hepatocyte-like cells (HLCs) with a four weeks protocol. RT-PCR, flow cytometry, IHC and ICC were performed to assess expression of key markers in both undifferentiated and differentiate…