MARCATORI BIOMOLECOLARI NELLE MALATTIE CRONICHE DEL FEGATO : IMPLICAZIONI DIAGNOSTICHE E TERAPEUTICHE

Cyclooxygenase- 2, Tumor Necrosis Factor-α, Vascular Endothelial Growth Factor-A and IL-6 genes SNPs and IL-6 serum levels in liver cirrhosis and hepatocellular carcinoma

Association between single nucleotide polymorphysms (SNPs) of IL-6, TNF-alpha and VEGF-A genes and susceptibility to hepatocellular carcinoma (HCC)

COX-2 gene CpG islands methylation status and SNP -765G>C as potential biomarkers of prognosis in pancreatic adenocarcinoma

Association between single nucleotide polymorphisms in the COX-2, TNF-alpha and VEGF-A genes and susceptibility to hepatocellular carcinoma

Association between single nucleotide polymorphisms in the COX-2, TNF-a and VEGF-A genes, and susceptibility to hepatocellular carcinoma

Introduction: TNF-a, COX-2 and Vascular Endothelial Growth Factor (VEGFA) are mediators of inflammation and angiogenesis, all of them are abundantly produced in liver cirrhosis (LC). It was proposed that there is an association between single nucleotide polymorphisms (SNPs) and hepatocellular carcinoma (HCC). These allelic variants influence the transcriptional activity of these genes, and therefore the proteins levels. The VEGF-A pathway is a potential therapeutic target in HCC, and several anti-angiogenic agents have entered clinical trials in HCC. Aims: 1) To evaluate thè frequency of SNPs of COX-2, TNF-a and VEGF-A genes in patients with HCC vs LC patients and a control group (C). 2) To…

CYP2E1 VNTR polymorphisms and hepatocarcinoma: a gender-specific correlation

Cytochrome P450 (CYP2E1) is often associate to susceptibility to alcohol-related diseases and various cancers, because of its role in the metabolism of multiple environmental xenobiotics. In the 5’- flanking region of the human CYP2E1 gene there are restriction fragment length polymorphism which are involved in the transcriptional regulation of the CYP2E1 gene. Recently a tandem repeat polymorphism (VNTR) in the 5’-flanking region of CYP2E1 was found. Because cytochrome P450 2E1 catalyzes the metabolic activation of pro-carcinogen and cytotoxic compound, we value the genetic distribution of this tandem repeat polymorphism in a healthy population, and in patients with hepatocellular carcinom…

Variable Number of Tandem Repeats (VNTR) gene polymorphism of CYP2E1 in patients with pancreatic adenocarcinoma

Context: The genetic polymorphism is considered a major source of variability, influencing the levels of gene expression. Cytochrome P450 2E1 (CYP2E1) is a mixed-function oxidase involved in the metabolism of the many endogenous and exogenous substances (ethanol, chemical carcinogens) in the hepatic and pancreatic tissue. CYP2E1 gene polymorphisms can cause various abilities of metabolize xenobiotic substances within a population with consequent increased susceptibility to various diseases,including cancer. One of the polymorphisms of the CYP2E1 gene is a VNTR (Variable Number Tandem Repeat) of some sequences in its "5 '- flanking region. Method : VNTR genotype CYP2E1 was determined by RFLP…

Genetic determined downregulation of both type 1 and type 2 cytokine pathways might be protective against pancreatic cancer

Association between single nucleotide polymorphisms (SNPs) of IL-6, TNF-alpha and VEGF-A genes, and susceptibility to hepatocellular carcinoma (HCC)

Background: Many studies have demonstrated the association between SNPs and susceptibility to the development of diseases such as autoimmune diseases and neoplasms. These allelic variations may involve regulatory and coding regions of cytokine genes and may influence their transcriptional activity and, as a consequence, their protein levels. Hepatocarcinogenesis is a multi-step and multi-factorial process in which both environmental and genetic factors are involved. Liver cirrhosis (LC) of both HBV or HCV origin is considered the most important risk factors for HCC. Objectives: We evaluated the frequency of genetic polymorphisms of the cytokines IL-6 and TNFalpha and of VEGF-A in a group of…