0000000000591782
AUTHOR
Ilva Pole
Genotype and allele frequencies of isoniazid-metabolizing enzymes NAT2 and GSTM1 in Latvian tuberculosis patients
Pharmacogenomic testing of tuberculosis drug-metabolizing enzyme genes was proposed as a strategy to identify patients at risk for suboptimal responses to medications. However, variations of the genotype frequencies among ethnic groups exist and new alleles are been identified. The aim of this study was to identify polymorphisms of genes encoding metabolic enzymes NAT2 and GSTM1 in tuberculosis patients in Latvia and to estimate the frequency of NAT2 slow acetylator and GSTM1 null genotypes. In total, 85 DNA samples were genotyped, all individuals were Caucasian. An ethnic heterogeneity reflecting the multiethnic population of the country was observed. 49 patients were Latvians, 30 were Rus…
Comparison of telomere length between population-specific mitochondrial haplogroups among different age groups in a Latvian population
Population studies have demonstrated that telomere length (TL) displays great diversity among different populations. Previously described controversial findings associated longevity with specific mitochondrial DNA haplogroups (hgs) (e.g., J and U). These observations may be influenced by population diversity, geographic location, and/or specific historic background. The aims of this study were to identify a specific hg which correlates with aging in a Latvian populating and to evaluate the possible association of TL variability with specific mitochondrial hgs. The results show no significant correlation between TL, mitochondrial DNA hgs and longevity. A slight increase in frequency was obse…
Insights into archaeological human sample microbiome using 16S rRNA gene sequencing
Human body is inhabited by a vast number of microorganisms, collectively known as human microbiome, and there is a tremendous interest in evolutionary changes of human microbial ecology, diversity and function. The field of paleomicrobiology – study of ancient human microbiome – is powered by modern techniques of Next Generation Sequencing (NGS), which allows extracting microbial genomic data directly from archaeological sample of interest. One of the major techniques is 16S rRNA gene sequencing, by which certain 16S rRNA gene hypervariable regions are being amplified and sequenced. However, some limitations of this method exist including taxonomic precision and efficacy of different region…
Analysis of the bacterial communities in ancient human bones and burial soil samples: Tracing the impact of environmental bacteria
Abstract In our attempts to reveal the hidden fragments of the history of the natural world, ancient DNA (aDNA) is the precious missing key that allows us to discover hidden truths about ourselves and the world around us. Not only does aDNA encrypt genetic data from a particular individual, it also carries information about the microbial communities that were present in the individual. However, the process of such data mining has many intrinsic challenges. One of the main challenges in aDNA research is the contamination of archaeological material with environmental bacteria from the surrounding soil and postmortem microbial sources. The goal of this study was to identify the microbial commu…
Mycobacterium tuberculosis Acquires Limited Genetic Diversity in Prolonged Infections, Reactivations and Transmissions Involving Multiple Hosts
9 páginas, 5 figuras y material suplementario en: https://www.frontiersin.org/articles/10.3389/fmicb.2017.02661/full#supplementary-material