0000000000591988

AUTHOR

Anna Maria Brucculeri

showing 2 related works from this author

Lower sperm DNA fragmentation after r-FSH administration in functional hypogonadotropic hypogonadism

2013

Abstract PURPOSE: An observational clinical and molecular study was designed to evaluate the effects of the administration of recombinant human FSH on sperm DNA fragmentation in men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. METHODS: In the study were included 53 men with a non-classical form of hypogonadotropic hypogonadism and idiopathic oligoasthenoteratozoospermia. In all patients, sperm DNA fragmentation index (DFI), assessed by terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate (dUTP) in situ DNA nick end-labelling (TUNEL) assay, was evaluated before starting the treatment with 150 IU of recombinant hum…

AdultMaleendocrine systemmedicine.medical_specialtyDNA FragmentationAsthenozoospermialaw.inventionAndrologyHypogonadotropic hypogonadismlawGamete BiologyInternal medicineGeneticsmedicineHumansApoptosis Gametogenesis DNA SpermSettore BIO/06 - Anatomia Comparata E CitologiaGenetics (clinical)GametogenesisSperm Countbusiness.industryHypogonadismObstetrics and GynecologyOligospermiaGeneral Medicinemedicine.diseaseSpermatozoaSpermRecombinant ProteinsEndocrinologyReproductive MedicineAsthenozoospermiaOligospermiaApoptosisRecombinant DNADNA fragmentationFollicle Stimulating Hormone HumanbusinessDevelopmental Biology
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New molecular markers for the evaluation of gamete quality.

2013

Purpose: Only 30 % of IVF cycles result in a pregnancy, so that multiple embryos need to be replaced, per treatment cycle, to increase pregnancy rates, resulting in a multiple gestation rate of 25 %. The use of new markers in the gamete selection, could reduce the number of the oocytes to be fertilized and embryos to be produced, but the tools to evidence the gamete competence remain unavailable and more studies are needed to identify bio-markers to select the best oocyte and sperm to produce embryos with higher implantation potentiality. Methods: To define oocyte competence, the apoptosis of the surrounding cumulus cells and the oxygen consumption rates for individual oocytes before fertil…

MaleOocytePregnancy RateApoptosisFertilization in VitroBiologyGerm CellArticleAndrologyHuman fertilizationGeneticCompetencePregnancyGeneticsmedicineApoptosis; Competence; Oocyte; Sperm; Apoptosis; Biological Markers; Chromatin; Cumulus Cells; Female; Fertilization in Vitro; Germ Cells; Humans; Male; Oocytes; Pregnancy; Pregnancy Rate; Sperm Injections Intracytoplasmic; Spermatozoa; Obstetrics and Gynecology; Reproductive Medicine; Developmental Biology; Genetics; Genetics (clinical)HumansSperm Injections IntracytoplasmicSettore BIO/06 - Anatomia Comparata E CitologiaGenetics (clinical)Cumulus CellPregnancyCumulus CellsApoptosiObstetrics and GynecologyEmbryoGeneral MedicineOocytemedicine.diseaseSpermSpermatozoaSpermChromatinChromatinPregnancy ratemedicine.anatomical_structureGerm CellsReproductive MedicineBiological MarkerOocytesGameteFemaleBiomarkersHumanDevelopmental BiologyJournal of assisted reproduction and genetics
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