0000000000597513
AUTHOR
Lucia Gimeno Mallench
showing 3 related works from this author
Activación del metabolismo energético del ratón mediante el tratamiento con resveratrol o genisteína.
2018
En los últimos años son muchos los estudios que se han realizado para evaluar las propiedades de los compuestos naturales en ciertas patologías, y como alguna de sus moléculas son capaces de modificar parámetros metabólicos en estados fisiopatológicos, causados por una enfermedad como la diabetes, obesidad e incuso cáncer, que tiene un gran componente metabólico. Es por ello que estudiar el efecto de los polifenoles, resveratrol o genisteína, puede ser de gran interés para comprender el mecanismo de acción mediante el cual inducen variaciones en el metabolismo. Son diversos los autores que han descrito que el resveratrol afecta el metabolismo energético y sirve como un mimético de restricci…
The importance of culturing primary cells under physiological conditions: proliferation, senescence, pluripotency
2017
Mesenchymal stem cells (MSCs), such as human dental pulp stem cells (hDPSCs), are currently a source for cell therapy. However, cell therapy protocols require 10–400 million cells per treatment, and consequently, they need to be expanded in vitro before implantation, with the inconvenience that MSCs undergo senescence following a certain number of cell expansion passages, loosing their stem cell qualities. Ambient oxygen tension (21% pO2) is normally used for in vitro culture, but physiological levels in vivo range between 3% and 6% pO2. We previously demonstrated that hDPSC proliferation rate is significantly lowered at 21% pO2 due to enhanced oxidative stress, which led to the activation …
Clearing Amyloid-β through PPARγ/ApoE Activation by Genistein is a Treatment of Experimental Alzheimer’s Disease
2016
Amyloid-b (Ab) clearance from brain, which is decreased in Alzheimer's disease, is facilitated by apolipoprotein E (ApoE). ApoE is upregulated by activation of the retinoid X receptor moiety of the RXR/PPAR dimeric receptor. As we have previously demonstrated, estrogenic compounds, such as genistein, have antioxidant activity, which can be evidenced by increased expression of manganese superoxide dismutase (MnSOD). Furthermore, genistein is a non-toxic, well-tested, and inexpensive drug that activates PPARg receptor. We isolated and cultured cortical astrocytes from dissected cerebral cortices of neonatal mice (C57BL/6 J). Preincubation with genistein (5 mM) for 24 hours, prior to the addit…