Species-specific polymerase chain reaction primers for simple detection of Bursaphelenchus fraudulentus (Nematoda: Parasitaphelenchidae)

Nematology , 2010, Vol. 12(1), 157-160 Short communication Species-specific polymerase chain reaction primers for simple detection of Bursaphelenchus fraudulentus (Nematoda: Parasitaphelenchidae) Anna F I L I P I A K 1 , ∗ , Agata J AKUBOW SKA 1 , 2 and Marek T OMALAK 1 Bursaphelenchus fraudulentus Ruhm, 1956 (Nema- toda: Parasitaphelenchidae) is a tree-inhabiting species frequently found in Europe (Ruhm, 1956; Schauer-Blume & Sturhan, 1989; Tomalak, 2004; Carletti et al ., 2005) and Asia (Braasch et al ., 2001). It is primarily found in dy- ing or dead deciduous trees (Ruhm, 1956; Schauer-Blume & Sturhan, 1989), although occasionally reported from conifers (Braasch et al ., 2001). Within t…