0000000000605840

AUTHOR

Tõnu Reintamm

showing 2 related works from this author

Identification of the reaction products of (2'-5')oligoadenylate synthetase in the marine sponge.

1998

Previously we reported on the presence of a high (2'-5')oligoadenylate synthetase activity in the marine sponge Geodia cydonium [Kuusksalu, A., Pihlak, A., Muller, W. E. G. & Kelve, M. (1995) Eur. J. Biochem. 232, 351-357]. The presence of (2'-5')oligoadenylates [(2'-5')A] in crude sponge extract was shown by radioimmunoassay and by their HPLC comigration with authentic (2'-5')A oligomers. In addition, the sponge (2'-5')oligoadenylates displayed biological activity, as determined by inhibition studies of protein biosynthesis in rabbit reticulocyte lysate. In the present study individual (2'-5')oligoadenylates synthesized by sponge enzyme were separated by HPLC. The exact composition of ever…

chemistry.chemical_classificationMagnetic Resonance Spectroscopy2'-5'-OligoadenylateOligonucleotideBiological activityNuclear magnetic resonance spectroscopyBiologyBiochemistryPoriferaEnzymemedicine.anatomical_structureBiochemistrychemistryReticulocyteSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationPhosphodiester bondProtein biosynthesismedicine2'5'-Oligoadenylate SynthetaseAnimalsChromatography High Pressure LiquidEuropean journal of biochemistry
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2′,5′-oligoadenylate synthetase from a lower invertebrate, the marine sponge Geodia cydonium, does not need dsRNA for its enzymatic activity

2002

AbstractRecently, the presence of 2′,5′-linked oligoadenylates and a high 2′,5′-oligoadenylate synthetase activity were discovered in a lower invertebrate, the marine sponge Geodia cydonium. It has been demonstrated that mammalian 2–5A synthetase isozymes require a dsRNA cofactor for their enzymatic activity. Our results show that, unlike mammalian 2–5A synthetases, the 2–5A synthetase from the sponge acts in a dsRNA-independent manner in vitro. A prolonged incubation of the G. cydonium extract with a high concentration of a micrococcal nuclease had no effect on the activity of the 2–5A synthetase. At the same time, the micrococcal nuclease was effective within 30 min in degrading dsRNA nee…

Gene isoformInterferon InducersGeodia cydoniumdsRNABiologyIsozymePC12 CellsCofactorSubstrate SpecificitySpecies SpecificitySponge2'5'-Oligoadenylate SynthetaseAnimalsMicrococcal Nuclease2–5A synthetaseMolecular BiologyRNA Double-Strandedchemistry.chemical_classificationOligoribonucleotidesEnzymatic activity2'-5'-OligoadenylateAdenine NucleotidesRNACell BiologyHydrogen-Ion ConcentrationEnzymes ImmobilizedIn vitroPoriferaRatsEnzymePoly I-CBiochemistrychemistrybiology.proteinMicrococcal nucleaseBiochimica et Biophysica Acta (BBA) - Molecular Cell Research
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