Human blastocysts derived from monopronuclear zygotes: a biological model for the study of ploidy, euploidy, topography and heteroparental inheritance

Rescuing monopronucleated-derived human blastocysts: a model to study chromosomal topography and fingerprinting.

Objective To quantify the percentage of monopronuclear-derived blastocysts (MNBs) that are potentially useful for reproductive purposes using classic and state-of-the-art chromosome analysis approaches, and to study chromosomal distribution in the inner cell mass (ICM) and trophectoderm (TE) for intertissue/intratissue concordance comparison. Design Prospective experimental study. Setting Single-center in vitro fertilization clinic and reproductive genetics laboratory. Patient(s) A total of 1,128 monopronuclear zygotes were obtained between June 2016 and December 2018. Intervention(s) MNBs were whole-fixed or biopsied to obtain a portion of ICM and 2 TE portions (TE1 and TE2) and were subse…

Effect of different 6-dimethylaminopurine (6-DMAP) treatments on reversible arresting of mono- and tripronuclear embryos at the pronuclear stage