0000000000614068
AUTHOR
Jesús Macías
Goodpasture Antigen-binding Protein Is a Soluble Exportable Protein That Interacts with Type IV Collagen
Goodpasture-antigen binding protein (GPBP) is a nonconventional Ser/Thr kinase for basement membrane type IV collagen. Various studies have questioned these findings and proposed that GPBP serves as transporter of ceramide between the endoplasmic reticulum and the Golgi apparatus. Here we show that cells expressed at least two GPBP isoforms resulting from canonical (77-kDa) and noncanonical (91-kDa) mRNA translation initiation. The 77-kDa polypeptide interacted with type IV collagen and localized as a soluble form in the extracellular compartment. The 91-kDa polypeptide and its derived 120-kDa polypeptide associated with cellular membranes and regulated the extracellular levels of the 77-kD…
Studying and improving Mathematics instruction in secondary schools in Spain study group (grupo de estudio SiMiS1 )
El objetivo de este artículo es presentar y justificar el diseño de POEMat.ES, una pauta de observación de acciones del profesor de matemáticas en clase. POEMat.ES ha sido desarrollado por un grupo de investigadores con perfiles diversos en cuanto a experiencia docente e investigadora, a fin de sistematizar la observación de la práctica de enseñanza para entender e interpretar con precisión el proceso de enseñanza de matemáticas en educación secundaria en el contexto español. El diseño de POEMat.ES se ha basado en modelos específicos del conocimiento del profesor de matemáticas. La construcción del instrumento se aborda desde dos aproximaciones complementarias: se proponen categorías de obs…
Goodpasture Antigen-binding Protein (GPBP) Directs Myofibril Formation
Goodpasture antigen-binding protein-1 (GPBP-1) is an exportable non-conventional Ser/Thr kinase that regulates glomerular basement membrane collagen organization. Here we provide evidence that GPBP-1 accumulates in the cytoplasm of differentiating mouse myoblasts prior to myosin synthesis. Myoblasts deficient in GPBP-1 display defective myofibril formation, whereas myofibrils assemble with enhanced efficiency in those overexpressing GPBP-1. We also show that GPBP-1 targets the previously unidentified GIP130 (GPBP-interacting protein of 130 kDa), which binds to myosin and promotes its myofibrillar assembly. This report reveals that GPBP-1 directs myofibril formation, an observation that expa…
Increased Goodpasture antigen-binding protein expression induces type IV collagen disorganization and deposit of immunoglobulin A in glomerular basement membrane
Increased expression of Goodpasture antigen-binding protein (GPBP), a protein that binds and phosphorylates basement membrane collagen, has been associated with immune complex-mediated pathogenesis. However, recent reports have questioned this biological function and proposed that GPBP serves as a cytosolic ceramide transporter (CERTL). Thus, the role of GPBP in vivo remains unknown. New Zealand White (NZW) mice are considered healthy animals although they convey a genetic predisposition for immune complex-mediated glomerulonephritis. Here we show that NZW mice developed age-dependent lupus-prone autoimmune response and immune complex-mediated glomerulonephritis characterized by elevated GP…