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RESEARCH PRODUCT
Goodpasture Antigen-binding Protein (GPBP) Directs Myofibril Formation
Ernesto López-pascualJesús MacíasJuan SausFernando RevertRoy ZentAnas SaadeddinBilly G. HudsonFroilán Granero-moltóFrancisco Revert-rosRichard M. BreyerCarmen NavarroRaúl Mínguez BlascoDeborah J. Burkssubject
Basement membraneEffectormacromolecular substancesCell BiologyBiologymusculoskeletal systemBiochemistryCell biologymedicine.anatomical_structureCytoplasmMyosinmedicineMyocyteCytoskeletonMyofibriltissuesMolecular BiologyIntracellulardescription
Goodpasture antigen-binding protein-1 (GPBP-1) is an exportable non-conventional Ser/Thr kinase that regulates glomerular basement membrane collagen organization. Here we provide evidence that GPBP-1 accumulates in the cytoplasm of differentiating mouse myoblasts prior to myosin synthesis. Myoblasts deficient in GPBP-1 display defective myofibril formation, whereas myofibrils assemble with enhanced efficiency in those overexpressing GPBP-1. We also show that GPBP-1 targets the previously unidentified GIP130 (GPBP-interacting protein of 130 kDa), which binds to myosin and promotes its myofibrillar assembly. This report reveals that GPBP-1 directs myofibril formation, an observation that expands its reported role in supramolecular organization of structural proteins to the intracellular compartment.
| year | journal | country | edition | language |
|---|---|---|---|---|
| 2011-10-01 | Journal of Biological Chemistry |