Quantitative analyse der selbstassoziation von hämoglobinmolekülen anhand der konzentrationsabhängigkeit der spektren

The dependence of the spectrum of oxygenated hemoglobin (Hb) on the Hb concentration was measured and it was found that Hb solutions do not obey Lambert-Beer's law. The absorption coefficient depends clearly on the Hb concentration. As all external parameters were kept constant except Hb concentration, the only way to explain our result is to assume that Hb molecules undergo self association and that this self association is reflected in the behaviour of the absorption coefficient. To get parameters which are suited to describe the self association of Hb molecules, two models were used. The first model is based on the idea that Hb associates according to the following equation: Hb + Hb ⇌ (H…

Excited state dipole moments and polarizabilities of centrosymmetric and dimeric molecules. I. Model study of a bichromophoric molecule

Abstract Refractometric, dielectric and electro-optical absorption measurements are reported for 1-dimethylamino-2,6-dicyano-4-methyl-benzene (I) and 1,4-bis(4′-dimethylamino-3′,5′-dicyanophenyl)bicyclo[2.2.2]octane (II). The evaluation leads to dipole moments and polarizabilities of the ground state as well as the first dipole allowed singlet state. The experimental res excellently substantiate the method of electro-optical absorption measurements in solution. It is shown that the excited dimer wavefunctions of the bichromophoric molecule II localize by solvent induced local site perturbations.

IS IT IMPORTANT TO SEPARATE LEUCOCYTES AND PLATELETS BEFORE MEASURING THE FILTERABILITY OF RED BLOOD CELLS?