0000000000617785
AUTHOR
J.l. Moreno Frigols
Influencia de la temperatura en las curvas de calibración en IRMA de enolasa neuronal específica y su interpretación fisicoquímica
Resumen Objetivo El analisis inmunorradiometrico (IRMA) es uno de los metodos principales utilizados en la determinacion analitica de la enolasa neuronal especifica (NSE). Se ha estudiado la influencia de la temperatura en las graficas de calibracion obtenidas por dicha tecnica y se propone una justificacion fisicoquimica basada en dos modelos teoricos. Material y metodos Usamos un kit comercial de IRMA para NSE y un contador gamma. Los resultados son analizados mediante el programa Statistica. Resultados y discusion Las actividades ligadas al anticuerpo aumentan con la temperatura, lo que da resultados que se ajustan a sendas modificaciones de las ecuaciones de los cuatro parametros y Lang…
Kinetics in a Double Antibody Radioimmunoassay (RIA): Diffussion Control
Competitive protein binding radioimmunoassay (CPB-RIA) is a principal method for quantifying serum C Peptide concentration. The accuracy of this method is critically dependent on factors that influence the reaction between anti-C Peptide antibody (P) with 125I-C Peptide (M). We studied the influence of initial concentration of M, ionic strength, and viscosity on the reaction between M and P. A kinetic model for the the reaction between. Such model adjusts satisfac- torily to the results. Bi-exponential and irreversible kinetics is determined. The results of the viscosity analysis show clear negative influence on the direct reaction rate. The ionic strength shows scarce influence on equilibr…
[99mTc]Ca-Phytate: Some colloidal characteristics related to the optimal preparation conditions
Abstract Some physico-chemical characteristics of the colloidal radiopharmaceutical [ 99 m Tc]Ca-phytate related to optimal preparation conditions have been studied. (1,2) It is demonstrated that the Ca 2+ -phytate stoichiometry is 6:1. Two different Ca-phytate colloids seem to be formed, mainly depending on the Ca 2+ :phytate molar ratio-one of low mycelar size for a 1:1 Ca 2+ :phytate molar ratio ( cmc ∗ = 5.10 −5 M ) , and another one, with a higher mycelar size for a 6:1 molar ratio (cmc = 8.10 −5 M). This last one it probably better for providing a good quality splenic uptake.
Kinetics and equilibrium in insulin radioimmunoassay.
The kinetics of insulin reaction has been studied with its specific antibody immobilized on the inner wall of the reaction tube; the radioimmunoanalytical determination of such a substance is based on the reaction. Independent variables were labelled and unlabelled insulin concentrations, temperature, viscosity, and the medium's ionic strength. Biexponential kinetics was found to be dependent on the concentrations fitted to the models discussed in the paper. The effect of temperature shows activation parameters similar to the viscous flow energy of water, which suggests that the reaction is diffusion-controlled. The results of the viscosity analysis points at the clearly negative influence …