Identification and phylogenetic classification of Rhizobium species by mapped restriction site polymorphism (MRSP) analysis of PCR-amplified 16S rRNA genes: Application to the characterization of communities isolated directly from soil samples and to the bias generated by analysis of mixed-cultures or mixed-16S rDNAS

Classification of rhizobia based on nodC and nifH gene analysis reveals a close phylogenetic relationship among Phaseolus vulgaris symbionts

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Phylogenetic comparison of symbiotic (nodC and nifH) and 16S rRNA genes in strains of Rhizobium, Mesorhizobium and Bradyrhizobium isolated from Astragalus, Oxytropis and Onobrychis spp.

Various studies reported different conclusions on the phylogenetic relationship between symbiotic (nod and nif) and chromosomal (16S rRNA) genes in rhizobia (Haukka et al, 1998). Some studies suggested that the phylogeny of nod genes could be related with the host plant (Ueda et al., 1995). In a previous study, rhizobia (44 isolates) from Astragalus, Oxytropis and Onobrychis pp have been classified into 14 distinct 16S rRNA gene types independently of their host plant and geographic origin. The majority of isolates were classified within the genus Mesorhizobium, and few within Rhizobium and Bradyrhizobium genera (Laguerre et al., 1997). The aim of the present study was to determine the dive…