0000000000623725

AUTHOR

M.i. Font-san-ambrosio

showing 2 related works from this author

Detection of Southern tomato virus by molecular hybridisation

2017

0301 basic medicine03 medical and health sciences030104 developmental biologySouthern tomato virusBiologyAgronomy and Crop ScienceVirologyAnnals of Applied Biology
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Fast detection of Southern tomato virus by one-step transcription loop-mediated isothermal amplification (RT-LAMP)

2017

Southern tomato virus (STV) is a double stranded RNA (dsRNA) virus belonging to genus Amalgavirus (family Amalgamaviridae) which has been detected in tomato plants showing stunting, fruit discoloration and size reduction. A one-step reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed for the detection of STV in total RNA or sap extracts (obtained just by grinding in buffer) from STV-infected tomato plants by using a set of three primers pairs which were designed to the sequence of the STV putative coat protein. Amplification products were visualized by gel electrophoresis or direct staining of DNA. The sensitivity of RT-LAMP was identical to that of th…

0301 basic medicineLoop-mediated isothermal amplificationSensitivity and SpecificityVirusPlant Viruses03 medical and health sciencesSolanum lycopersicumTranscription (biology)VirologyPlant virusPolymeraseDNA PrimersPlant DiseasesGel electrophoresisbiologyfungiTemperaturefood and beveragesReverse TranscriptionNucleic acid amplification techniqueVirologyMolecular biologyRNA silencing030104 developmental biologybiology.proteinRNA ViralNucleic Acid Amplification TechniquesJournal of Virological Methods
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