Immobilization of <i>Saccharomyces cerevisiae</i> Cells to Protein G-Sepharose by Cell Wall Engineering

In this work, we explored the possibility of using the targeting of a heterologous protein to the cell wall of <i>Saccharomyces cerevisiae</i>, by fusing it to a cell wall protein, to construct yeast strains whose cells display on their surface proteins that bind to a matrix, so as to achieve the immobilization of the whole cells. With this aim, we created a gene fusion that comprises the region responsible for attachment of a cell wall protein to the cell wall, and the IgG binding region of staphylococcal protein A, and expressed it in the <i>mnn1mnn9</i> strain of <i>S. cerevisiae</i>. The surface display of the protein A-Icwp fusion protein was positiv…

Translational fusion to the Pir4 cell wall protein as a general and efficient method for cell surface immobilization or growth medium secretion of recombinant antigens and enzymes in Saccharomyces cerevisiae