0000000000626495
AUTHOR
Reinis Ornicāns
Using Anther Culture Method for Flax Breeding Intensification
Flax breeding is a long and complicated process based on hybridization and following selection of the best plants. Because of possible occasional cross-pollination the development of genetically stable homozygous lines could last more than 15 years. For more rapid creating of initial material for flax breeding anther culture methods for producing doubled haploid (DH) lines could be used successfully. The goal of this study was to develop the best anther culture protocol for producing DH lines from hybrids included in Latvian flax breeding programme and to do preliminary field evaluation of obtained DH lines. F4 hybrids were used in the experiment. Method, most applicable for establishing of…
Anther Culture Effectiveness in Producing Doubled Haploids of Cereals/ Putekðòu Kultûras Efektivitâte Graudaugu Dubultoto Haploîdu Izveidoðanâ
Abstract Our goal was to improve the method of obtaining double haploids (DH) by anther culture from Latvian breeding material for several agriculturally important cereals in Latvia: barley, spring and winter wheat. Hybrids from Latvian breeders were initial material. It was found that copper (2.5 mg/l) added to barley anther pre-treatment media and to both barley (C3) and wheat (liquid AMC) induction media, resulted in a higher percentage of green plants-regenerants. In general, the winter wheat hybrids exhibited lower embryogenesis than spring wheat hybrids. Obtaining a large number of DH lines from barley and wheat hybrids with unknown androgenesis response can be organized in two stages…
Baltijas plakangliemenes Macoma balthica populācijas ģenētiskās daudzveidības analīze Rīgas jūras līča piekrastē
Bakalaura darbā pētīta baltijas plakangliemeņu (Macoma balthica) populācijas ģenētiskā daudzveidība Rīgas jūras līča piekrastes zonā. Izmantojot iPBS (inter - Primer Binding Sequences) metodi veikta 12 dažādu atradņu gliemeņu ģenētiskās daudzveidības analīze. PCR amplifikācijas produkti, pēc reakcijas ar diviem praimeriem (2232 un 2394), tika analizēti agarozes gēlā. Kopā tika konstatēti 38lokusi. Darbā analizētajiem paraugiem konstatēts augsts polimorfisma līmenis. Salīdzinot atradnes savā starpā, polimorfu lokusu skaits - 22 (59 %). Ģenētiskā līdzība visām populācijām ir augstāka par 88 %. Iegūtie dati tika matemātiski apstrādāti ar POPGENE un NTSYS programmnodrošinājumu.
Genetic Diversity of Liparis loeselii in Latvia
Abstract According to the Global Strategy for Plant Conservation, 75% of endangered species should be preserved in ex situ collections till 2020. The genus Liparis has a lot of recognised taxons, but only one species, Liparis loeselii (L.) Rich., grows in Europe. L. loeselii is a rare and endangered orchid species occurring in Europe. In Latvia L. loeselii is classified as the third category of endangered and protected species. To develop the best conservation strategy, the knowledge concerning the genetic differences of protected plants in a particular area is crucial. For this purpose, the genetic diversity of L. loeselii populations from different Latvian habitats was tested. The inter-r…