MALDI-TOF mass spectrometry identification of filamentous fungi in the clinical laboratory.

Pôle MERS F. Dalle; International audience; This study aimed to validate the effectiveness of a standardised procedure for the MALDI-TOF mass spectrometry (MS)-based identification on a large sample of filamentous fungi routinely identified in university hospitals' laboratories. Non-dermatophyte filamentous fungi prospectively isolated in the routine activity of five teaching hospitals in France were first identified by conventional methods in each laboratory and then by MS in one centre. DNA sequence-based identification resolved discrepancies between both methods. In this study, of the 625 analysed filamentous fungi of 58 species, 501 (80%) and 556 (89%) were correctly identified by conve…

Construction, validation et amélioration continue de banque de spectres pour l’utilisation en routine de la spectrométrie de masse en mycologie médicale

Résumé publié dans: Journal de Mycologie Médicale / Journal of Medical Mycology, sept. 2012, 55(3): 279-280. doi:10.1016/j.mycmed.2012.07.024; National audience

Évaluation multicentriquede l’identification des champignons filamenteux par la spectrométrie de masse MALDI-TOF

New ECCO model documents for Material Deposit and Transfer Agreements in compliance with the Nagoya Protocol

The European Culture Collections Organisation presents two new model documents for Material Deposit Agreement (MDA) and Material Transfer Agreement (MTA) designed to enable microbial culture collection leaders to draft appropriate agreement documents for, respectively, deposit and supply of materials from a public collection. These tools provide guidance to collections seeking to draft an MDA and MTA, and are available in open access to be used, modified, and shared. The MDA model consists of a set of core fields typically included in a deposit form to collect relevant information to facilitate assessment of the status of the material under access and benefit sharing (ABS) legislation. It a…

Comparison of MALDI-TOF mass spectra with microsatellite length polymorphisms inCandida albicans

Candida albicans is the most frequent yeast involved in human infections. Its population structure can be divided into several genetic clades, some of which have been associated with antifungal susceptibility. Therefore, detecting and monitoring fungal clones in a routine laboratory setting would be a major epidemiological advance. Matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) mass spectra results are now widely used as bar codes to identify microorganisms in clinical microbiology laboratories. This study aimed at testing MALDI-TOF mass spectra bar codes to identify clades among a set of C. albicans isolates. Accordingly, 102 clinical strains were genotyped using 10…