0000000000635426
AUTHOR
Jie Pan
Excitation-Wavelength-Dependent Photocycle Initiation Dynamics Resolve Heterogeneity in the Photoactive Yellow Protein from Halorhodospira halophila
Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore this question by examining the excitation-wavelength dependence of the photodynamics of the PYP from Halorhodospira halophila via a combined experimental and computational approach. The fluorescence quantum yield, steady-state fluorescence emission maximum, and cryotrapping spectra are demonstrated to depend on excitation wavelength. We also compare the femtosecond photodynamics …
Excitation-Wavelength Dependent Photocycle Initiation Dynamics Resolve Heterogeneity in the Photoactive Yellow Protein from Halorhodospira halophila
Photoactive yellow proteins (PYPs) make up a diverse class of blue-light-absorbing bacterial photoreceptors. Electronic excitation of the p-coumaric acid chromophore covalently bound within PYP results in triphasic quenching kinetics; however, the molecular basis of this behavior remains unresolved. Here we explore this question by examining the excitation-wavelength dependence of the photodynamics of the PYP from Halorhodospira halophila via a combined experimental and computational approach. The fluorescence quantum yield, steady-state fluorescence emission maximum, and cryotrapping spectra are demonstrated to depend on excitation wavelength. We also compare the femtosecond photodynamics …
Study of light backgrounds from relativistic electrons in air light-guides
The MOLLER experiment proposed at the Thomas Jefferson National Accelerator Facility plans a precision low energy determination of the weak mixing angle via the measurement of the parity-violating asymmetry in the scattering of high energy longitudinally polarized electrons from electrons bound in a liquid hydrogen target (M{\o}ller scattering). A relative measure of the scattering rate is planned to be obtained by intercepting the M{\o}ller scattered electrons with a circular array of thin fused silica tiles attached to air light guides, which facilitate the transport of Cherenkov photons generated within the tiles to photomultiplier tubes (PMTs). The scattered flux will also pass through …