0000000000640051
AUTHOR
Philippe Usseglio-polatera
Investigating the emerging role of comparative proteomics in the search for new biomarkers of metal contamination under varying abiotic conditions
13 pages; International audience; This study aims at investigating the potential use of comparative proteomics as a multi-marker approach of metal contamination, taking into account the potential confounding effect of water temperature. The major objective was to identify combinations of proteins specifically responding to a given metal, even if included in a metal mixture. The diagnostic approach was performed via the comparative analysis of protein expression on spot mapping provided by adult males of Gammarus pulex (Amphipoda, Crustacea) respectively exposed to arsenate (As), cadmium (Cd) or a binary mixture of these metals (AsCd) at three realistic temperatures (5, 10 and 15 °C). Proteo…
Development and dissemination to operational stakeholders of integrative tools for chemical and biological measurements in watercourses to monitor the impact of pesticides according to agricultural practices and their evolution
The results of the Impact-CE project highlight the specific contribution of various integrative tools to have a more complete vision of the chemical and biological impact of pesticides on watercourses. In addition to grabe sampling, which gives a quantitative and instantaneous view of contamination, these tools provide more representative information over time, which makes them better tools for prioritising basins that contribute to contamination. They are also more robust for reporting interannual changes in agricultural practices, even if it may remain difficult to dissociate the effect of limited changes in practices from that of significant climatic variations. The results also confirm …