0000000000644246
AUTHOR
Talat Tariq Khalil
Complexation des acides aminés basiques arginine, histidine et lysine avec l’ADN plasmidique en solution aqueuse : participation à la capture de radicaux sous irradiation X à 1,5 keV
L’environnement chimique de l’ADN en situation biologique est complexe notam-ment en raison de la presence d’histones, proteines nucleaires, associees en quantite approximativement egales a l’ADN pour former la chromatine. Les histones possedent de nombreux radicaux basiques arginine et lysine charges positivement et dont la majorite se trouve sur les chaines emergentes, l’ADN presente quant a lui des charges negatives sur ses groupements phosphates localises tout au long de la double helice. Dans cette etude, la complexite de la structure de la chromatine nucleaire est dans un premier temps mimee en solution aqueuse par la formation de complexes entre un ADN plasmidique sonde et les trois …
The contribution of hydrogen peroxide to the radiosensitizing effect of gold nanoparticles
Abstract Plasmid DNA in aerated aqueous solution is used as a probe to determine whose of the reactive oxygen species (ROS) generated after absorption of ultra-soft X-rays (USX) take part in biomolecule damage in the presence and in absence of Gold Nano-Particles (GNP) and specific scavengers. Citrate-coated GNPs with core sizes of 6, 10 and 25 nm are synthetized and characterized, especially in terms of plasmon band shift, ζ-potential and hydrodynamic radii (respectively 9, 21 and 30 nm). We confirm the radiosensitizing effect of GNP and show that the SSB number per plasmid increases when, for a same mass of gold element, the core size of the gold nanoparticles decreases. Hydroxyl radicals…
Auto-assembly of nanometer thick, water soluble layers of plasmid DNA complexed with diamines and basic amino acids on graphite: Greatest DNA protection is obtained with arginine.
Abstract We have investigated the ability of diamines as well as basic amino acids to condense DNA onto highly ordered pyrolytic graphite with minimum damage after re-dissolution in water. Based on a bibliographic survey we briefly summarize DNA binding properties with diamines as compared to basic amino acids. Thus, solutions of DNA complexed with these linkers were drop-cast in order to deposit ultra-thin layers on the surface of HOPG in the absence or presence of Tris buffer. Atomic Force Microscopy analyses showed that, at a fixed ligand-DNA mixing ratio of 16, the mean thickness of the layers can be statistically predicted to lie in the range 0–50 nm with a maximum standard deviation ±…
Single- and Double-Strand Breaks of Dry DNA Exposed to Protons at Bragg-Peak Energies
International audience; Ultrathin layers (<20 nm) of pBR322 plasmid DNA were deposited onto 2.5 μm thick polyester films and exposed to proton Bragg-peak energies (90–3000 keV) at various fluences. A quantitative analysis of radio-induced DNA damage is reported here in terms of single- and double-strand breaks (SSB and DSB, respectively). The corresponding yields as well as G-values and the cross sections exhibit fairly good agreement with the rare available data, stemming from close experimental conditions, namely, based on α particle irradiation. SSB/DSB rates appear to be linear when plotted against linear energy transfer (LET) in the whole energy range studied. All the data present a ma…