0000000000646714

AUTHOR

Francesca Perrone

showing 3 related works from this author

Galactosylated polyaspartamide copolymers for siRNA targeted delivery to hepatocellular carcinoma cells

2017

The limited efficacy of available treatments for hepatocellular carcinoma (HCC) requires the development of novel therapeutic approaches. We synthesized a novel cationic polymer based on α,β-poly-(N-2-hydroxyethyl)-D,L-aspartamide (PHEA) for drug delivery to HCC cells. The copolymer was synthesized by subsequent derivatization of PHEA with diethylene triamine (DETA) and with a polyethylene glycol (PEG) derivative bearing galactose (GAL) molecules, obtaining the cationic derivative PHEA-DETA-PEG-GAL. PHEA-DETA-PEG-GAL has suitable chemical-physical characteristics for a potential systemic use and can effectively deliver a siRNA (siE2F1) targeted against the transcription factor E2F1, a gen…

Polyplexes HCC siRNA E2F1 PHEA-DETA-PEG-GALCarcinoma HepatocellularPolymersPharmaceutical ScienceE2F1; HCC; PHEA-DETA-PEG-GAL; Polyplexes; siRNA.02 engineering and technologyPolyethylene glycol03 medical and health scienceschemistry.chemical_compound0302 clinical medicineCell Line TumorPEG ratiomedicineHumansE2F1Gene silencingGene SilencingRNA Small InterferingHCCReceptorCell growthChemistryLiver NeoplasmssiRNA.021001 nanoscience & nanotechnologymedicine.diseaseMolecular biologyPHEA-DETA-PEG-GALPolyplexeE2F1030220 oncology & carcinogenesisHepatocellular carcinomasiRNADrug deliveryCancer researchPeptides0210 nano-technologyE2F1 Transcription FactorPolyplexes
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Recent advances in smart biotechnology: Hydrogels and nanocarriers for tailored bioactive molecules depot

2017

Over the past ten years, the global biopharmaceutical market has remarkably grown, with ten over the top twenty worldwide high performance medical treatment sales being biologics. Thus, biotech R&D (research and development) sector is becoming a key leading branch, with expanding revenues. Biotechnology offers considerable advantages compared to traditional therapeutic approaches, such as reducing side effects, specific treatments, higher patient compliance and therefore more effective treatments leading to lower healthcare costs. Within this sector, smart nanotechnology and colloidal self-assembling systems represent pivotal tools able to modulate the delivery of therapeutics. A comprehens…

Bioactive molecules02 engineering and technologyHepatocellular-carcinoma cells01 natural sciencesMiceColloid and Surface ChemistryDrug Delivery SystemsCarbon nano materialNanotechnologyMolecular Targeted TherapyTransgenesRNA Small InterferingPatient complianceTransfer radical polymerizationMicro/nanocarrierMedical treatmentMicro/nanocarriersBioactive molecule deliveryHydrogelsSurfaces and Interfaces021001 nanoscience & nanotechnologyLiposomeBiopharmaceuticalOral deliverySelf-healing hydrogelsIntercellular Signaling Peptides and Proteins0210 nano-technologyAssembling peptide hydrogelsSurfaces and InterfaceNucleic-acid deliveryPlasmidsDiagnostic ImagingSolid lipid nanoparticlesNanotechnology010402 general chemistryAntibodiesSmall Molecule LibrariesCarbon nano-onionsIn-vivoAnimalsHumansPhysical and Theoretical Chemistrybusiness.industryDrug-delivery0104 chemical sciencesBiotechnologyHydrogelSettore CHIM/09 - Farmaceutico Tecnologico ApplicativoMolecular ProbesBioactive molecule delivery; Carbon nano materials; Hydrogels; Liposomes; Micro/nanocarriers; Surfaces and Interfaces; Physical and Theoretical Chemistry; Colloid and Surface ChemistryLiposomesNonviral gene deliveryCarbon nano materialsNanoparticlesBusinessNanocarriers
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Targeted delivery of siRNAs against hepatocellular carcinoma-related genes by a galactosylated polyaspartamide copolymer

2021

Given the lack of effective treatments for Hepatocellular carcinoma (HCC), the development of novel therapeutic approaches is very urgent. Here, siRNAs were delivered to HCC cells by a synthetic polymer containing α,β-poly-(N-2-hydroxyethyl)-D,L-aspartamide-(PHEA) derivatized with diethylene triamine (DETA) and bearing in the side chain galactose (GAL) linked via a polyethylene glycol (PEG) to obtain (PHEA-DETA-PEG-GAL, PDPG). The GAL residue allows the targeting to the asialo-glycoprotein receptor (ASGPR), overexpressed in HCC cells compared to normal hepatocytes. Uptake studies performed using a model siRNA or a siRNA targeted against the enhanced green fluorescence protein, demonstrated …

Small interfering RNACarcinoma HepatocellularPolymersHepatocellular carcinomaCellASGPR targeted delivery; E2F1; Eukaryotic elongation Factor 1A; Hepatocellular carcinoma; siRNAPharmaceutical Science02 engineering and technologyEukaryotic elongation Factor 1AMice03 medical and health sciencesIn vivomedicineAnimalsE2F1RNA Small InterferingReceptor030304 developmental biology0303 health sciencesChemistryLiver NeoplasmsASGPR targeted deliveryGalactose021001 nanoscience & nanotechnologymedicine.diseasedigestive system diseasesEukaryotic translation elongation factor 1 alpha 1In vitromedicine.anatomical_structureE2F1Settore CHIM/09 - Farmaceutico Tecnologico ApplicativoHepatocellular carcinomasiRNACancer research0210 nano-technology
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