0000000000661120

AUTHOR

Fabrizio Torricelli

showing 3 related works from this author

Monitoring of Cell Layer Integrity with a Current-Driven Organic Electrochemical Transistor

2019

Abstract The integrity of CaCo-2 cell barriers is investigated by organic electrochemical transistors (OECTs) in a current-driven configuration. Ion transport through cellular barriers via the paracellular pathway is modulated by tight junctions between adjacent cells. Rupturing its integrity by H2O2 is monitored by the change of the output voltage in the transfer characteristics. It is demonstrated that by operating the OECT in a current-driven configuration, the sensitive and temporal resolution for monitoring the cell barrier integrity is strongly enhanced as compared to the OECT transient response measurement. As a result, current-driven OECTs are useful tools to assess dynamic and crit…

Materials scienceTransistors Electroniccell barriersBiomedical EngineeringPharmaceutical ScienceBiosensing Techniques02 engineering and technologybioelectronics010402 general chemistry01 natural scienceslaw.inventionBiomaterialslawElectrochemistryHumansTransient responseinvertersCell ShapeIon transporterBioelectronicsTight junctionbioelectronics; cell barriers; inverters; organic electrochemical transistors; toxicologybusiness.industryTransistorHydrogen Peroxide021001 nanoscience & nanotechnologyorganic electrochemical transistors0104 chemical sciencesParacellular transportOptoelectronicsCaco-2 Cells0210 nano-technologybusinesstoxicologyVoltageOrganic electrochemical transistor
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Current-Driven Organic Electrochemical Transistors for Monitoring Cell Layer Integrity with Enhanced Sensitivity

2021

In this progress report an overview is given on the use of the organic electrochemical transistor (OECT) as a biosensor for impedance sensing of cell layers. The transient OECT current can be used to detect changes in the impedance of the cell layer, as shown by Jimison et al. To circumvent the application of a high gate bias and preventing electrolysis of the electrolyte, in case of small impedance variations, an alternative measuring technique based on an OECT in a current-driven configuration is developed. The ion-sensitivity is larger than 1200 mV V-1 dec-1 at low operating voltage. It can be even further enhanced using an OECT based complementary amplifier, which consists of a p-type a…

Materials scienceTransistors ElectronicBiomedical EngineeringPharmaceutical ScienceElectrolyteBiosensing TechniquesTransistorslaw.inventionBiomaterialsElectrolytesPEDOT:PSSimpedance sensinglawcell layer integrityElectric ImpedanceElectronicPEDOT:PSSHumansElectrical impedanceorganic bioelectronicsElectrolysisbusiness.industryAmplifierTransistorcell layer integrity; impedance sensing; organic bioelectronics; organic electro-chemical transistors; PEDOT:PSS; Caco-2 Cells; Electric Impedance; Electrolytes; Humans; Biosensing Techniques; Transistors ElectronicOptoelectronicsCaco-2 Cellsbusinessorganic electro-chemical transistorsBiosensorOrganic electrochemical transistor
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Monitoring Reversible Tight Junction Modulation with a Current-Driven Organic Electrochemical Transistor

2021

Materials scienceTight junctionbusiness.industrybiosensors; organic electrochemical transistor; poly- l-lysine; reversible tight junction modulationbiosensorsIndustrial and Manufacturing Engineeringpoly- l-lysineorganic electrochemical transistorMechanics of MaterialsModulationreversible tight junction modulationOptoelectronicsGeneral Materials ScienceCurrent (fluid)businessBiosensorOrganic electrochemical transistor
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